"
Team:Wageningen UR/Protocol
From 2012.igem.org
(Difference between revisions)
m |
|||
| Line 68: | Line 68: | ||
<li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/Frenchpress|French press user manual]]</li> | ||
<li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | <li>[[Team:Wageningen_UR/Protocol/FPLC|FPLC user manual]]</li> | ||
| + | <li>[[Team:Wageningen_UR/Protocol/Mutagenesis|Mutagenesis]]</li> | ||
</ul> | </ul> | ||
Latest revision as of 10:44, 26 October 2012
Contents |
Methods
The use of Virus-Like-Particles as medicine carrier is new for iGEM. This means the whole production, purification and detection of Virus-Like-Particles is also new in iGEM. In this section we will explain how the different methods work and how it all fits together.
Protocols
Medium & Buffer recipes
- LB medium
- SOB medium
- SOC medium
- Disassembly buffer
- Dialysis buffer 10x
- Reassembly buffer
- Virus buffer
- Formation Buffer HepBcAg
- Washing Buffer HepBcAg
- Formation Buffer Polero
- Towbin's electrotransfer buffer 1x
- Towbin's electrotransfer buffer 10x
CCMV Coat Protein VLP formation
Hepatitis B Coat Protein VLP formation
Polerovirus Coat Protein VLP formation
General Protocol
- Preparation of electrocompetent cells of E. coli
- SDS-Polyacrylamide and native gel electrophoresis
- Dynamic Light Scattering user manual
- French press user manual
- FPLC user manual
- Mutagenesis
