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Team:UT Dallas/npopulation toolkit
From 2012.igem.org
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<td align='left' valign='top' width="15px" style='background:transparent'>44</td><td align='left' valign='top' width="305px" style='background:transparent'>pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR</td><td align='center' valign='top' style="width:100px;background:url('https://static.igem.org/mediawiki/2012/4/4f/All.png') transparent no-repeat top"><a href='http://partsregistry.org/Part:BBa_K876073'>BBa_K876073</a></td><td align='left' valign='top' width="45px" style='background:transparent'>Repressor/ Reporter/ Signaling/ Reciever</td><td align='left' valign='top' width="305px" style='background:transparent'>Recieves las and luxR. Produces Ai-2 quorum signaling molecule. CFP reporter. Represses pTet.</td></tr></table> | <td align='left' valign='top' width="15px" style='background:transparent'>44</td><td align='left' valign='top' width="305px" style='background:transparent'>pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR</td><td align='center' valign='top' style="width:100px;background:url('https://static.igem.org/mediawiki/2012/4/4f/All.png') transparent no-repeat top"><a href='http://partsregistry.org/Part:BBa_K876073'>BBa_K876073</a></td><td align='left' valign='top' width="45px" style='background:transparent'>Repressor/ Reporter/ Signaling/ Reciever</td><td align='left' valign='top' width="305px" style='background:transparent'>Recieves las and luxR. Produces Ai-2 quorum signaling molecule. CFP reporter. Represses pTet.</td></tr></table> | ||
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| + | <center><table border="0" style="width:770px;background: none repeat scroll 0 0 transparent;"> | ||
<tr> | <tr> | ||
<td style="width:60px;background: url('https://static.igem.org/mediawiki/2012/0/0f/Repressor.png') repeat-y scroll center top transparent;"></td> | <td style="width:60px;background: url('https://static.igem.org/mediawiki/2012/0/0f/Repressor.png') repeat-y scroll center top transparent;"></td> | ||
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<td>Intermediate</td> | <td>Intermediate</td> | ||
</tr> | </tr> | ||
| - | </table> | + | </table></center> |
Revision as of 03:54, 4 October 2012
Toolkit
| No | Part | Registry ID | Function | Description |
| 1 | pLac-TetR | <a href='http://partsregistry.org/Part:BBa_K876000'>BBa_K876000</a> | Repressor | Represses pTet promoter |
| 2 | pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP | <a href='http://partsregistry.org/Part:BBa_K876001'>BBa_K876001</a> | Reporter/ Repressor | Will lock into one of two different states when induced with the chemicals IPTG or ATc./RFP and GFP reporter |
| 3 | pTet-LacI | <a href='http://partsregistry.org/Part:BBa_K876002'>BBa_K876002</a> | Repressor | Represses plac promoter |
| 4 | pLac-RBS-GFP-T | <a href='http://partsregistry.org/Part:BBa_K876003'>BBa_K876003</a> | Reporter | GFP reporter |
| 5 | pTet-RBS-GFP-T-pLac-TetR | <a href='http://partsregistry.org/Part:BBa_K876004'>BBa_K876004</a> | Reporter/ Repressor | Negative feedback with GFP reporter - TetR represses ptet promoter |
| 6 | pLac-RBS-GFP-T-pTet-LacI | <a href='http://partsregistry.org/Part:BBa_K876005'>BBa_K876005</a> | Reporter/ Repressor | Negative feedback with GFP reporter - LacI represses plac promoter |
| 7 | RFP-LVA | <a href='http://partsregistry.org/Part:BBa_K876006'>BBa_K876006</a> | Reporter | RFP reporter |
| 8 | pLac-LacO | <a href='http://partsregistry.org/Part:BBa_K876007'>BBa_K876007</a> | Intermediate | plac with two lac operator sites |
| 9 | pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI | <a href='http://partsregistry.org/Part:BBa_K876008'>BBa_K876008</a> | Reciever/ Reporter/ Signaling/ Repressor | Recieves luxR. Represses Ptet. Produces Ai-1 quorum signaling molecule. GFP Reporter |
| 10 | LuxR-DT-pLux-TetR | <a href='http://partsregistry.org/Part:BBa_K876009'>BBa_K876009</a> | Reciever/ Repressor | Recieves lux R. Represses pTet. |
| 11 | pLux-TetR | <a href='http://partsregistry.org/Part:BBa_K876010'>BBa_K876010</a> | Reciever/ Repressor | Recieves luxR. Represses pTet. |
| 12 | pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI | <a href='http://partsregistry.org/Part:BBa_K876011'>BBa_K876011</a> | Reciever/ Repressor/ Signaling/ Reporter | Recieves lasR. Represses plac. Produces AHL quorum signaling molecule.RFP reporter |
| 13 | pLas-LacI-RFP-ST-pLac-LuxI | <a href='http://partsregistry.org/Part:BBa_K876012'>BBa_K876012</a> | Reciever/ Repressor/ Signaling/ Reporter | Recieves lasR. Represses plac. Produces AHL quorum signaling molecule.RFP reporter |
| 14 | LacI-RFP-ST-pLac-LuxI | <a href='http://partsregistry.org/Part:BBa_K876013'>BBa_K876013</a> | Repressor/ Reporter | Represses plac. RFP reporter |
| 15 | pLac-LuxI | <a href='http://partsregistry.org/Part:BBa_K876014'>BBa_K876014</a> | Signaling | Produces AHL quorum signaling molecule |
| 16 | pTet-LasR-DT | <a href='http://partsregistry.org/Part:BBa_K876015'>BBa_K876015</a> | Intermediate | Intermediate |
| 17 | plac-lacO-RBS-GFP-T-ptet-Laci | <a href='http://partsregistry.org/Part:BBa_K876016'>BBa_K876016</a> | Reporter/ Repressor | GFP reporter. Represses plac. |
| 18 | plac-lacOX2-RBS-GFP-T-ptet-Laci | <a href='http://partsregistry.org/Part:BBa_K876017'>BBa_K876017</a> | Reporter/ Repressor | GFP reporter. Represses plac. |
| 21 | TT-pLasR-araC | <a href='http://partsregistry.org/Part:BBa_K876038'>BBa_K876038</a> | Reciever/ Repressor | Recieves lasR. Represses pBad. |
| 22 | TT-pLasR-LuxS-RBS-CFP | <a href='http://partsregistry.org/Part:BBa_K876039'>BBa_K876039</a> | Signaling/ Reciever/ Reporter | Recieves lasR. Produces Ai-2 quorum sensing molecule. CFP reporter. |
| 23 | TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR | <a href='http://partsregistry.org/Part:BBa_K876040'>BBa_K876040</a> | Signaling/ Reciever/ Reporter/ Repressor | Recieves lasR. Produces Ai-2 quorum sensing molecule. CFP reporter. Represses pTet |
| 24 | TT-pLsrA-LuxI-RBS-YFP | <a href='http://partsregistry.org/Part:BBa_K876041'>BBa_K876041</a> | Signaling/ Reciever/ Reporter | Recieves Ai-2. Produces AHL quorum sensing molecule. YFP reporter. |
| 25 | pLasR-araC | <a href='http://partsregistry.org/Part:BBa_K876043'>BBa_K876043</a> | Reciever/ Repressor | Recieves lasR. Represses pBad. |
| 26 | lasI-RBS-RFP | <a href='http://partsregistry.org/Part:BBa_K876046'>BBa_K876046</a> | Signaling/ Reporter | Produces Ai-1 quorum sensing molecule. RFP reporter. |
| 27 | pBad-LsrK-TT-pLsrA-LuxI-YFP (Pop 1 Oscillator) | <a href='http://partsregistry.org/Part:BBa_K876047'>BBa_K876047</a> | Signaling/ Reciever/ Reporter | Produces AHL quorum sensing molecule. Recieves Ai-2 molecule. YFP reporter. |
| 28 | plac-lasI-RBS-RFP (pop2 AND GATE) | <a href='http://partsregistry.org/Part:BBa_K876048'>BBa_K876048</a> | Signaling/ Reporter | Produces Ai-1 quorum sensing molecule. RFP reporter. |
| 29 | plasR-luxI-RBS-YFP | <a href='http://partsregistry.org/Part:BBa_K876049'>BBa_K876049</a> | Signaling/ Reciever/ Reporter | Recieves lasR. Produces AHL quorum sensing molecule. RFP reporter. |
| 30 | pTetR-lasR-TT | <a href='http://partsregistry.org/Part:BBa_K876057'>BBa_K876057</a> | Intermediate | Intermediate |
| 31 | TT-plasR-RBS-CFP | <a href='http://partsregistry.org/Part:BBa_K876060'>BBa_K876060</a> | Reciever/ Reporter | Recieves lasR. CFP reporter. |
| 32 | TT-pluxR-LasI-RBS-RFP | <a href='http://partsregistry.org/Part:BBa_K876061'>BBa_K876061</a> | Reciever/ Reporter/ Signaling | Produces Ai-1 quorum sensing molecule. RFP reporter. Recieves luxR. |
| 33 | pTetR-luxI | <a href='http://partsregistry.org/Part:BBa_K876062'>BBa_K876062</a> | Signaling | Produces AHL quorum sensing molecule. RFP reporter. |
| 34 | luxI-RBS-YFP | <a href='http://partsregistry.org/Part:BBa_K876063'>BBa_K876063</a> | Signaling/ Reporter | Produces AHL quorum sensing molecule. RFP reporter. |
| 35 | plsrA-luxI-RBS-YFP | <a href='http://partsregistry.org/Part:BBa_K876064'>BBa_K876064</a> | Signaling/ Reporter/ Reciever | Produces AHL quorum sensing molecule. YFP reporter. Recieves Ai-2 molecule. |
| 36 | pTetR-lasR-TT-plasR-luxI-RBS-YFP (Pop 1 AND GATE) | <a href='http://partsregistry.org/Part:BBa_K876065'>BBa_K876065</a> | Signaling/ Reporter/ Reciever | Produces AHL quorum sensing molecule. YFP reporter. Recieves lasR molecule. |
| 37 | plac-luxR-TT-pluxR-araC-lasR-TT-pBad-tetR-TT-plasR.TetO-RBS-CFP (pop 3 AND GATE) | <a href='http://partsregistry.org/Part:BBa_K876066'>BBa_K876066</a> | Repressor/ Reporter/ Reciever | arac rpresses pBad and TetR represses pTet. Recieves lasR and luxR. CFP reporter |
| 38 | TT-pLac-LuxR | <a href='http://partsregistry.org/Part:BBa_K876067'>BBa_K876067</a> | Intermediate | Intermediate |
| 39 | TT-pLasrA-LacI | <a href='http://partsregistry.org/Part:BBa_K876068'>BBa_K876068</a> | Reciever | Recieves Ai-2 molecule |
| 40 | TT-pLuxR-TetR | <a href='http://partsregistry.org/Part:BBa_K876069'>BBa_K876069</a> | Reciever/ Repressor | Recieves luxR. Represses pTet |
| 41 | CP-GFP | <a href='http://partsregistry.org/Part:BBa_K876070'>BBa_K876070</a> | Reporter | GFP reporter |
| 42 | pLac-TetR-RBS-RFP-pTetR-LacI-RBS-GFP | <a href='http://partsregistry.org/Part:BBa_K876071'>BBa_K876071</a> | Repressor/ Reporter | RFP and GFP reporter. Rpresesses pTet and plac |
| 43 | pLac-TetR-RBS-RFP | <a href='http://partsregistry.org/Part:BBa_K876072'>BBa_K876072</a> | Repressor/ Reporter | Represses pTet. RFPreporter |
| 44 | pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR | <a href='http://partsregistry.org/Part:BBa_K876073'>BBa_K876073</a> | Repressor/ Reporter/ Signaling/ Reciever | Recieves las and luxR. Produces Ai-2 quorum signaling molecule. CFP reporter. Represses pTet. |
| Repressor | Reporter | Signaling | Receiver | Intermediate |
