Team:UT Dallas/npopulation toolkit

From 2012.igem.org

Toolkit

NoPartRegistry IDFunctionDescription
1pLac-TetR<a href='http://partsregistry.org/Part:BBa_K876000'>BBa_K876000</a>RepressorRepresses pTet promoter
2pTet-LacI-RBS-GFP(LVA)-T-T-pLac-TetR-RBS-RFP<a href='http://partsregistry.org/Part:BBa_K876001'>BBa_K876001</a>Reporter/ RepressorWill lock into one of two different states when induced with the chemicals IPTG or ATc./RFP and GFP reporter
3pTet-LacI<a href='http://partsregistry.org/Part:BBa_K876002'>BBa_K876002</a>RepressorRepresses plac promoter
4pLac-RBS-GFP-T<a href='http://partsregistry.org/Part:BBa_K876003'>BBa_K876003</a>ReporterGFP reporter
5pTet-RBS-GFP-T-pLac-TetR<a href='http://partsregistry.org/Part:BBa_K876004'>BBa_K876004</a>Reporter/ RepressorNegative feedback with GFP reporter - TetR represses ptet promoter
6pLac-RBS-GFP-T-pTet-LacI<a href='http://partsregistry.org/Part:BBa_K876005'>BBa_K876005</a>Reporter/ RepressorNegative feedback with GFP reporter - LacI represses plac promoter
7RFP-LVA<a href='http://partsregistry.org/Part:BBa_K876006'>BBa_K876006</a>ReporterRFP reporter
8pLac-LacO<a href='http://partsregistry.org/Part:BBa_K876007'>BBa_K876007</a>Intermediateplac with two lac operator sites
9pLac-LuxR-DT-pLux-TetR-GFP-ST-pTet-LasI<a href='http://partsregistry.org/Part:BBa_K876008'>BBa_K876008</a>Reciever/ Reporter/ Signaling/ RepressorRecieves luxR. Represses Ptet. Produces Ai-1 quorum signaling molecule. GFP Reporter
10LuxR-DT-pLux-TetR<a href='http://partsregistry.org/Part:BBa_K876009'>BBa_K876009</a>Reciever/ RepressorRecieves lux R. Represses pTet.
11pLux-TetR<a href='http://partsregistry.org/Part:BBa_K876010'>BBa_K876010</a>Reciever/ RepressorRecieves luxR. Represses pTet.
12pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI<a href='http://partsregistry.org/Part:BBa_K876011'>BBa_K876011</a>Reciever/ Repressor/ Signaling/ ReporterRecieves lasR. Represses plac. Produces AHL quorum signaling molecule.RFP reporter
13pLas-LacI-RFP-ST-pLac-LuxI<a href='http://partsregistry.org/Part:BBa_K876012'>BBa_K876012</a>Reciever/ Repressor/ Signaling/ ReporterRecieves lasR. Represses plac. Produces AHL quorum signaling molecule.RFP reporter
14LacI-RFP-ST-pLac-LuxI<a href='http://partsregistry.org/Part:BBa_K876013'>BBa_K876013</a>Repressor/ ReporterRepresses plac. RFP reporter
15pLac-LuxI<a href='http://partsregistry.org/Part:BBa_K876014'>BBa_K876014</a>SignalingProduces AHL quorum signaling molecule
16pTet-LasR-DT<a href='http://partsregistry.org/Part:BBa_K876015'>BBa_K876015</a>IntermediateIntermediate
17plac-lacO-RBS-GFP-T-ptet-Laci<a href='http://partsregistry.org/Part:BBa_K876016'>BBa_K876016</a>Reporter/ RepressorGFP reporter. Represses plac.
18plac-lacOX2-RBS-GFP-T-ptet-Laci<a href='http://partsregistry.org/Part:BBa_K876017'>BBa_K876017</a>Reporter/ RepressorGFP reporter. Represses plac.
21TT-pLasR-araC<a href='http://partsregistry.org/Part:BBa_K876038'>BBa_K876038</a>Reciever/ RepressorRecieves lasR. Represses pBad.
22TT-pLasR-LuxS-RBS-CFP<a href='http://partsregistry.org/Part:BBa_K876039'>BBa_K876039</a>Signaling/ Reciever/ ReporterRecieves lasR. Produces Ai-2 quorum sensing molecule. CFP reporter.
23TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR<a href='http://partsregistry.org/Part:BBa_K876040'>BBa_K876040</a>Signaling/ Reciever/ Reporter/ RepressorRecieves lasR. Produces Ai-2 quorum sensing molecule. CFP reporter. Represses pTet
24TT-pLsrA-LuxI-RBS-YFP<a href='http://partsregistry.org/Part:BBa_K876041'>BBa_K876041</a>Signaling/ Reciever/ ReporterRecieves Ai-2. Produces AHL quorum sensing molecule. YFP reporter.
25pLasR-araC<a href='http://partsregistry.org/Part:BBa_K876043'>BBa_K876043</a>Reciever/ RepressorRecieves lasR. Represses pBad.
26lasI-RBS-RFP<a href='http://partsregistry.org/Part:BBa_K876046'>BBa_K876046</a>Signaling/ ReporterProduces Ai-1 quorum sensing molecule. RFP reporter.
27pBad-LsrK-TT-pLsrA-LuxI-YFP (Pop 1 Oscillator)<a href='http://partsregistry.org/Part:BBa_K876047'>BBa_K876047</a>Signaling/ Reciever/ ReporterProduces AHL quorum sensing molecule. Recieves Ai-2 molecule. YFP reporter.
28plac-lasI-RBS-RFP (pop2 AND GATE)<a href='http://partsregistry.org/Part:BBa_K876048'>BBa_K876048</a>Signaling/ ReporterProduces Ai-1 quorum sensing molecule. RFP reporter.
29plasR-luxI-RBS-YFP<a href='http://partsregistry.org/Part:BBa_K876049'>BBa_K876049</a>Signaling/ Reciever/ ReporterRecieves lasR. Produces AHL quorum sensing molecule. RFP reporter.
30pTetR-lasR-TT<a href='http://partsregistry.org/Part:BBa_K876057'>BBa_K876057</a>IntermediateIntermediate
31TT-plasR-RBS-CFP<a href='http://partsregistry.org/Part:BBa_K876060'>BBa_K876060</a>Reciever/ ReporterRecieves lasR. CFP reporter.
32TT-pluxR-LasI-RBS-RFP<a href='http://partsregistry.org/Part:BBa_K876061'>BBa_K876061</a>Reciever/ Reporter/ SignalingProduces Ai-1 quorum sensing molecule. RFP reporter. Recieves luxR.
33pTetR-luxI<a href='http://partsregistry.org/Part:BBa_K876062'>BBa_K876062</a>SignalingProduces AHL quorum sensing molecule. RFP reporter.
34luxI-RBS-YFP<a href='http://partsregistry.org/Part:BBa_K876063'>BBa_K876063</a>Signaling/ ReporterProduces AHL quorum sensing molecule. RFP reporter.
35plsrA-luxI-RBS-YFP<a href='http://partsregistry.org/Part:BBa_K876064'>BBa_K876064</a>Signaling/ Reporter/ RecieverProduces AHL quorum sensing molecule. YFP reporter. Recieves Ai-2 molecule.
36pTetR-lasR-TT-plasR-luxI-RBS-YFP (Pop 1 AND GATE)<a href='http://partsregistry.org/Part:BBa_K876065'>BBa_K876065</a>Signaling/ Reporter/ RecieverProduces AHL quorum sensing molecule. YFP reporter. Recieves lasR molecule.
37plac-luxR-TT-pluxR-araC-lasR-TT-pBad-tetR-TT-plasR.TetO-RBS-CFP (pop 3 AND GATE)<a href='http://partsregistry.org/Part:BBa_K876066'>BBa_K876066</a>Repressor/ Reporter/ Recieverarac rpresses pBad and TetR represses pTet. Recieves lasR and luxR. CFP reporter
38TT-pLac-LuxR<a href='http://partsregistry.org/Part:BBa_K876067'>BBa_K876067</a>IntermediateIntermediate
39TT-pLasrA-LacI<a href='http://partsregistry.org/Part:BBa_K876068'>BBa_K876068</a>RecieverRecieves Ai-2 molecule
40TT-pLuxR-TetR<a href='http://partsregistry.org/Part:BBa_K876069'>BBa_K876069</a>Reciever/ RepressorRecieves luxR. Represses pTet
41CP-GFP<a href='http://partsregistry.org/Part:BBa_K876070'>BBa_K876070</a>ReporterGFP reporter
42pLac-TetR-RBS-RFP-pTetR-LacI-RBS-GFP<a href='http://partsregistry.org/Part:BBa_K876071'>BBa_K876071</a>Repressor/ ReporterRFP and GFP reporter. Rpresesses pTet and plac
43pLac-TetR-RBS-RFP<a href='http://partsregistry.org/Part:BBa_K876072'>BBa_K876072</a>Repressor/ ReporterRepresses pTet. RFPreporter
44pTetR-LasR-TT-pLasR-LuxS-RBS-CFP-TT-pLuxR-TetR<a href='http://partsregistry.org/Part:BBa_K876073'>BBa_K876073</a>Repressor/ Reporter/ Signaling/ RecieverRecieves las and luxR. Produces Ai-2 quorum signaling molecule. CFP reporter. Represses pTet.


Repressor Reporter Signaling Receiver Intermediate