Team:Cornell/testing/project/wetlab/4/7

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<h3>Registry of Standard Parts Submitted</h3>
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<groupparts>iGEM012 Cornell</groupparts>
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<h3>First Lessons Learned: Control Reactors</h3>
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In order to enhance the field-deployability of our final device, we initially decided to feed our reactors with LB, since a very concentrated LB source fed at a low flow rate could sustain our field reactors for extended periods of time without taking up much physical space. However, upon setting up control reactors&#8212;both in batch and continuous flow operation&#8212;we discovered that wild type <i>S. oneidensis</i> MR-1 produced significantly less current when fed with LB than M4&#8212;a commonly used media for <i>Shewanella</i>-inoculated bioelectrochemical systems.
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<h3>Naphthalene & Salicylate Sensing</h3>
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First characterized naphthalene sensors with salicylate, since our system indirectly senses naphthalene via salicylate.
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Uninduced salicylate reporter produces same current as MR-1 in rich M4 media.
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First attempt to diagnose problem: Switch to minimal M4... Producing higher current, but we're still not getting a salicylate response.
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Next attempt: Poise the working electrode at a higher potential in attempt to get more MtrC and MtrA for MtrB to associate with. Didn't quite do the trick either.
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<h3>Arsenic Sensing</h3>
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Revision as of 03:44, 4 October 2012

Registry of Standard Parts Submitted

NameTypeDescriptionDesignerLength
    BBa_K957000GeneratorArsenic inducible mtrB with cut sites flanking RBSDylan Patrick Webster2625
    BBa_K957001GeneratorArsenic inducible mtrBDylan Patrick Webster2704
    BBa_K957002GeneratorSalicylate inducible mtrB with cut sites flanking RBS Dylan Patrick Webster3207
    BBa_K957003GeneratorSalicylate inducible mtrBDylan Patrick Webster3284
    BBa_K957004Codingnah Operon for degradation of naphthalene to salicylateDylan Patrick Webster9409
    BBa_K957005Generator Constitutive expression of nah Operon for degradation of naphthalene to salicylate Dylan Patrick Webster9452
  BBa_K957006CompositeConstitutive expression of nah Operon with oriT Dylan Patrick Webster9831

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