Team:Michigan/Notebook/Protocols
From 2012.igem.org
(Difference between revisions)
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Removing BioBricks from the Registry Wells[[File:MichRem.pdf]] | Removing BioBricks from the Registry Wells[[File:MichRem.pdf]] | ||
- | Production of Competent Cells | + | Production of Competent Cells[[File:MichRem.pdf|1]] |
Heat Shock Transformation | Heat Shock Transformation |
Revision as of 02:06, 4 October 2012
Protocols
General
Plate ProductionCloning
Removing BioBricks from the Registry WellsFile:MichRem.pdfProduction of Competent CellsFile:MichRem.pdf
Heat Shock Transformation
DNA Purification
Restriction Enzyme Digest
Ligation
PCR (Phusion)
Colony PCR
Characterization
Transmission Electron Microscopy ProtocolFimE Asymmetrical Digest Assay Protocol