From 2012.igem.org
(Difference between revisions)
|
|
| Line 242: |
Line 242: |
| | </p><p><br /> | | </p><p><br /> |
| | </p> | | </p> |
| - | <table id="toc" class="toc"><tr><td><div id="toctitle"><h2>Contents</h2></div>
| + | |
| - | <ul>
| + | |
| - | <li class="toclevel-1 tocsection-1"><a href="#Human Practices"><span class="tocnumber">1</span> <span class="toctext">Project Overview</span></a></li>
| + | |
| - | <li class="toclevel-1 tocsection-2"><a href="#Background"><span class="tocnumber">2</span> <span class="toctext">Background</span></a>
| + | |
| - | <ul>
| + | |
| - | <li class="toclevel-2 tocsection-3"><a href="#Limitations_of_Current_Detection_Methods"><span class="tocnumber">2.1</span> <span class="toctext">Limitations of Current Detection Methods</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-4"><a href="#Bacteriophage_Basics"><span class="tocnumber">2.2</span> <span class="toctext">Bacteriophage Basics</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-5"><a href="#Bordetella_Phage"><span class="tocnumber">2.3</span> <span class="toctext">Bordetella Phage</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-6"><a href="#T7_Phage"><span class="tocnumber">2.4</span> <span class="toctext">T7 Phage</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-7"><a href="#Human_Chorionic_Gonadotropin"><span class="tocnumber">2.5</span> <span class="toctext">Human Chorionic Gonadotropin</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-8"><a href="#Pregnancy_Tests"><span class="tocnumber">2.6</span> <span class="toctext">Pregnancy Tests</span></a></li>
| + | |
| - | </ul>
| + | |
| - | </li>
| + | |
| - | <li class="toclevel-1 tocsection-9"><a href="#Methods"><span class="tocnumber">3</span> <span class="toctext">Methods</span></a></li>
| + | |
| - | <ul>
| + | |
| - | <li class="toclevel-2 tocsection-10"><a href="#Sequence_Design"><span class="tocnumber">3.1</span> <span class="toctext">Sequence Design</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-11"><a href="#Isolation_of_Phage_and_Phage_Genome"><span class="tocnumber">3.2</span> <span class="toctext">Isolation of Phage and Phage Genome</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-12"><a href="#Cloning"><span class="tocnumber">3.3</span> <span class="toctext">Cloning</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-13"><a href="#Phage_Modification"><span class="tocnumber">3.4</span> <span class="toctext">Phage Modification</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-14"><a href="#Folding_Solution"><span class="tocnumber">3.5</span> <span class="toctext">Folding Solution</span></a></li>
| + | |
| - | </ul>
| + | |
| - | <li class="toclevel-1 tocsection-15"><a href="#Results"><span class="tocnumber">4</span> <span class="toctext">Results</span></a></li>
| + | |
| - | <ul>
| + | |
| - | <li class="toclevel-2 tocsection-16"><a href="#Phage_Production"><span class="tocnumber">4.1</span> <span class="toctext">Phage Production</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-17"><a href="#BioBrick_Confirmation"><span class="tocnumber">4.2</span> <span class="toctext">BioBrick Confirmation</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-18"><a href="#Wild-Type_hCG"><span class="tocnumber">4.3</span> <span class="toctext">Wild-Type hCG</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-19"><a href="#synCG_Device"><span class="tocnumber">4.4</span> <span class="toctext">synCG Device</span></a></li>
| + | |
| - | <li class="toclevel-2 tocsection-20"><a href="#Folding_Solution"><span class="tocnumber">4.5</span> <span class="toctext">Folding Solution</span></a></li>
| + | |
| - | </ul>
| + | |
| - | <li class="toclevel-1 tocsection-21"><a href="#Conclusions"><span class="tocnumber">5</span> <span class="toctext">Conclusions</span></a></li>
| + | |
| - | <li class="toclevel-1 tocsection-22"><a href="#References"><span class="tocnumber">6</span> <span class="toctext">References</span></a></li>
| + | |
| - | </ul>
| + | |
| - | </td></tr></table><script>if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script>
| + | |
| | <h2><span class="editsection"></span> <span class="mw-headline" id="Project_Overview"> <b>Attributions</b> </span></h2> | | <h2><span class="editsection"></span> <span class="mw-headline" id="Project_Overview"> <b>Attributions</b> </span></h2> |
| | | | |
Revision as of 01:56, 4 October 2012
Attributions
Siva Dasa, Ph.D. for his expertise in bacterial cultures, bacteriophage amplification, and bacteriophage isolation and many other laboratory techniques.
Jeff Smith, Ph.D. for his guidance in all aspects of molecular biology and genetic engineering on this project.
Gina Donato, Ph.D. for use of her BSL-2 lab space for experiments with B. pertussis, as well as her mentorship in all aspects related to Bordetella.
Kimberly Kelly, Ph.D. for hosting the entire iGEM team this year in her laboratory space and for helping us work through larger conceptual issues regarding the trajectory of the project
Eric Hewlett, Ph.D. for his expertise in epidemiology of Bordetella, and sharing his extensive network of scientific contacts in order to help us achieve our goals.
Drs. Keith Kozminski, Jason Papin, and Inchan Kwon for their continued mentorship of the Virginia iGEM team and their reliability in helping us navigate all things iGEM.
Arjun Athreya, member of Virginia iGEM 2010 and 2011, for guidance and feedback throughout the spring semester and early summer.
Yanzhi Yang, member of Virginia iGEM 2011 team, for useful conversations and helping with cell culture in the early summer.
Special thanks to the Kelly lab specialists and graduate students, including Jeff Smith and Stephanie Thomas, Lindsey Brinton, Marc Seaman, Soo Shin, and Jaymes Beech, for their constant guidance and support.
Sponsors
- Prof. Cahen - Alumni Lacey Fund
- Deans Hawley and Galloway
- UVA Medical School
- Kelly Laboratory
"
