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Team:Cornell/testing/project/wetlab/1
From 2012.igem.org
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| - | <h3> | + | <h3>Overview</h3> |
| - | + | We began the summer by holding a synthetic biology bootcamp at the DeLisa Lab. The purpose of this bootcamp was both to introduce new members to techniques in molecular biology and to get a running start on the cloning work for our project. During bootcamp, we successfully constructed both versions of our arsenic reporter, and attempted a Gibson assembly of a naphthalene-degrading plasmid. | |
| - | + | <br><br> | |
| + | In late June, we transitioned from bootcamp to our permanent bench space in Dr. Archer’s lab in Weill Hall. After spending a few weeks setting up the lab space troubleshooting general issues, we successfully constructed both versions of our salicylate reporter and began an alternative approach to construct a plasmid with a naphthalene-degrading operon. In parallel, we realized that electroporation efficiency for Shewanella transformation is less than optimal—to say the least. However, we were able to conjugate our constructs into Shewanella using a protocol provided by Dr. Gralnick. | ||
| + | <br><br> | ||
| + | As we transitioned into the fall semester, wetlab work was divided into ‘task forces’. | ||
| + | <br><br><ul> | ||
| + | <li>Site Directed mutagenesis</li> | ||
| + | <li>Western blotting</li> | ||
| + | <li>qPCR</li> | ||
| + | <li>Nah operon into Shewanella</li> | ||
| + | <li>Running reactors</li> | ||
| + | <li>Artificial River Media</li> | ||
| + | </ul> | ||
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Revision as of 00:58, 4 October 2012
-
Wet Lab
- Overview
- Chassis
- DNA Assembly
- Testing & Results
- Future Work
- Animation
Wet Lab Overview
Overview
We began the summer by holding a synthetic biology bootcamp at the DeLisa Lab. The purpose of this bootcamp was both to introduce new members to techniques in molecular biology and to get a running start on the cloning work for our project. During bootcamp, we successfully constructed both versions of our arsenic reporter, and attempted a Gibson assembly of a naphthalene-degrading plasmid.In late June, we transitioned from bootcamp to our permanent bench space in Dr. Archer’s lab in Weill Hall. After spending a few weeks setting up the lab space troubleshooting general issues, we successfully constructed both versions of our salicylate reporter and began an alternative approach to construct a plasmid with a naphthalene-degrading operon. In parallel, we realized that electroporation efficiency for Shewanella transformation is less than optimal—to say the least. However, we were able to conjugate our constructs into Shewanella using a protocol provided by Dr. Gralnick.
As we transitioned into the fall semester, wetlab work was divided into ‘task forces’.
- Site Directed mutagenesis
- Western blotting
- qPCR
- Nah operon into Shewanella
- Running reactors
- Artificial River Media
