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Team:uOttawa CA/Results
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Revision as of 03:51, 3 October 2012
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A current problem with designing synthetic networks is a lack of ability to externally control the system. By integrating and optimizing bacterial systems in S. cerevisiae the uOttawa team hopes to generate a new inducible system that can be used for synthetic gene network design in yeast.
Combinatorial Mating and Gene Regulatory Systems
An advantage of using yeast as a model organism is its ability to exist in both haploid and diploid states. The uOttawa team plans to utilize this ability to combinatorially mate haploid strains to build gene networks. A haploid strain that acts as reporter for gene regulatory proteins can be used as a "testing" strain and mated with other haploid "sample" strains to quickly and efficiently test gene regulatory functions.
Shuttle Vector
By building an E.coli/S.cerevisiae shuttle vector we can take advantage of the high reproductive rate of E.coli and the gene synthesis capabilities of S.cerevisiae. Networks will be built via homologous recombination in yeast and replicated in bacteria. Traditional drug selection will be supplemented with colour selection to increase the accuracy of the transformations.
Combinatorial Mating and Gene Regulatory Systems
An advantage of using yeast as a model organism is its ability to exist in both haploid and diploid states. The uOttawa team plans to utilize this ability to combinatorially mate haploid strains to build gene networks. A haploid strain that acts as reporter for gene regulatory proteins can be used as a "testing" strain and mated with other haploid "sample" strains to quickly and efficiently test gene regulatory functions.
Shuttle Vector
By building an E.coli/S.cerevisiae shuttle vector we can take advantage of the high reproductive rate of E.coli and the gene synthesis capabilities of S.cerevisiae. Networks will be built via homologous recombination in yeast and replicated in bacteria. Traditional drug selection will be supplemented with colour selection to increase the accuracy of the transformations.
