Team:Ehime-Japan/Safety

A1
No. Our project ideas do not raise safety issues in terms of them. We used the light sensor genes from Synechocystis and the lon and the ssrAtag genes from Mesoplasma florum in E.co-mail and E.co-Domino. Also, the lux and las gene regularion systems were used in E.cold-heat sensing system. An explanation of the safety standard about our experiments is as follows.
In Japan, we must document our local committee which organism the gene is from and what the host isbefore we carry out gene recombination experiments. The organisms that the gene used in our projects are originated from are shown in Table1 with the Class as defined in Japanese ordinance that the organism are classified into. The hosts used in our experiments are shown in Table 2.
※Class1: All the organisms expect class 2to4.
Class2:The organisms having low virulence to mammals.
Classes3and4 can cause more risks.
The organisms we are working with are E.coli strains DH5α,JT2(EnvZ),and JW0429(lon)and they have no virulence. Thus,they are all in Class1.According to the Japanese regulations, the containment measure(measure to be taken to prevent disposal of LMOs)to be taken should be determined primarily considering the Classes of the donor and host organisms.However,if the gene to be inserted into the host is already characterized to have no relation to the virulence or transmissibility of the organism from which the gene is originated,then,the containment measure can be determined only from the host Class.

In our case,Pseudomonas is in Class2,but the las regulation system by itself has no relation to the virulence:the system does not contain the lasBgene,which is regulated by the las system in the organism and causes the virulence.We also planned to use the samples from Mycoplasma capricolum,which are in Class2,though we did not use them. The situations are the same as in the case of Pseudomonas. All the other genes also have nothing to do with virulence to mammals. Therefore,the level of the containment measure to be taked to prevent dispersal of LMOs is P1,as determined from the Class of the host.Our documents were examined in our local committee and accepted without rasing any problem.Thus,we are doiing all experiments under the condition of P1 level experiment.
Table3.The P1 level containment measures to prevent dispersal of LMOs.Wecan conclude,therefore,that our project ideas are safe for researcher,public,and the environment.