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Team/CINVESTAV-IPN-UNAM MX/Construction.htm
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<p><strong>CHARACTERIZATION OF IGEM BIOPARTS DISTRIBUTION</strong></p> | <p><strong>CHARACTERIZATION OF IGEM BIOPARTS DISTRIBUTION</strong></p> | ||
<p>As part of the points required to perform well in 2012 iGEM competition we are asked to characterize some existing biopartes on the record, so we decided to make the characterization of constitutive promoters belonging to the family isolated from a small combinatorial library (J23101 , J23102, J23104, J23107, J23108, J2311, and J23115) which were attached to GFP to determine promoter activity, using the equipment Victor X3 Multilabel Plate Reader.</p> | <p>As part of the points required to perform well in 2012 iGEM competition we are asked to characterize some existing biopartes on the record, so we decided to make the characterization of constitutive promoters belonging to the family isolated from a small combinatorial library (J23101 , J23102, J23104, J23107, J23108, J2311, and J23115) which were attached to GFP to determine promoter activity, using the equipment Victor X3 Multilabel Plate Reader.</p> | ||
| - | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2012/3/3f/Co1.jpg" width="640" height="186" /></p> |
<p>Fig 1. General scheme of one construction of the promoter expressing GFP</p> | <p>Fig 1. General scheme of one construction of the promoter expressing GFP</p> | ||
| - | <p><img src=" | + | <p><img src="https://static.igem.org/mediawiki/2012/6/6b/Cota.jpg" width="511" height="271" /></p> |
<p><img src="images/Co2.jpg" width="640" height="470" /></p> | <p><img src="images/Co2.jpg" width="640" height="470" /></p> | ||
<p>Fig 2. Final construction</p> | <p>Fig 2. Final construction</p> | ||
Revision as of 03:16, 27 September 2012
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 CHARACTERIZATION OF IGEM BIOPARTS DISTRIBUTION As part of the points required to perform well in 2012 iGEM competition we are asked to characterize some existing biopartes on the record, so we decided to make the characterization of constitutive promoters belonging to the family isolated from a small combinatorial library (J23101 , J23102, J23104, J23107, J23108, J2311, and J23115) which were attached to GFP to determine promoter activity, using the equipment Victor X3 Multilabel Plate Reader.
Fig 1. General scheme of one construction of the promoter expressing GFP
Fig 2. Final construction
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