Team:Ehime-Japan/Modeling

From 2012.igem.org

(Difference between revisions)
Line 130: Line 130:
<b><font size="3">
<b><font size="3">
E.coli (pIJ106b, pJT122, plPCB) is cultivated in TY culture medium for 7 hours. It was precipitated by centrifugation and washed with 1 % NaClaq.
E.coli (pIJ106b, pJT122, plPCB) is cultivated in TY culture medium for 7 hours. It was precipitated by centrifugation and washed with 1 % NaClaq.
-
400 &mu;L of 1% NaCl was added to the E.coli sample and protein synthesis was stopped by addition of tetracyclin at the concentration of 100 &mu;g/mL.
+
400 &mu;L of 1% NaCl was added to the E.coli sample.
An aliquot from each sample was plated on agar and cultured overnight.  
An aliquot from each sample was plated on agar and cultured overnight.  
The solution was exposed to blue LED light, and we took a color picture every 1 hour for 6 hours.  
The solution was exposed to blue LED light, and we took a color picture every 1 hour for 6 hours.  

Revision as of 03:14, 27 September 2012

Ehime-Japan iGEM Team: Welcome


Introduction

In our experiment of the E.co-mail, the important thing is to degrade GFP-Lon-tag more rapidly. GFP is usually stable and has a long half–life. So, we thought of how to measure the reaction rate.

Method

E.coli (pIJ106b, pJT122, plPCB) is cultivated in TY culture medium for 7 hours. It was precipitated by centrifugation and washed with 1 % NaClaq. 400 μL of 1% NaCl was added to the E.coli sample. An aliquot from each sample was plated on agar and cultured overnight. The solution was exposed to blue LED light, and we took a color picture every 1 hour for 6 hours. By using the ImageJ software, we measured the intensity of light from GFP.

Retrieved from "http://2012.igem.org/Team:Ehime-Japan/Modeling"