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Team:Evry/Notebook/w15
From 2012.igem.org
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| - | <li>following that cloning the reporters downstream to various promotors was done | + | <li>following that cloning the reporters downstream to various promotors was done </li> |
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| + | <li> cloning og IAAh and IAAM in both Psb1C3 and Pcs2+ by standard biobrick portocol </li> | ||
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| + | <li> Golden gate assembly of IAAM-IAAH cassette in both Psb1C3 and Pcs2+ </li> | ||
<li>Injection in tadpoles for the parts we managed to clone and was validated correctly by sequencing </li> | <li>Injection in tadpoles for the parts we managed to clone and was validated correctly by sequencing </li> | ||
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| + | </ul> | ||
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Revision as of 01:22, 27 September 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 15: 17th September - 23rd September
{{:Team:Evry/template_notebook}}this week we have worked to characterize Promotors and Reporters in order to test their validity by injection in tadpoles :
- biobricking promotors in Pcs2+ which are then to be characterized in tadpoles after cloning upstream to various reporters including mCFP, SfGFP, and citrine which are:
- P Elastase
- Hsp70
- P Car A
- FLk1
- P CMV
