Team:SUSTC-Shenzhen-B/lab results

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Result

1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.

(Figure 1: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the RFP. )

(Figure 2: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

2. Fit curves relating to dscores and terminator efficiencies.

(figure 3 : According the data on biofab ( http://io.biofab.org/services/studio/dac/ ) and our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )

(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )

(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)

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-                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.   Pictures seen under fluorescence microscope</font></h3>
+                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.   Fluorescence microscope results</font></h3>
+Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.
                  <p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/d/d2/71.png" class="img_fl img_border" //font></p><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br>
-               <p><span class="STYLE3">(figure 1: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
+               <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
   <p class="STYLE2"><font face="Arial, Helvetica"><img src=" https://static.igem.org/mediawiki/2012/e/ec/81.png" class="img_fl img_border" //font></p><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br><Br>
-               <p><span class="STYLE3">(figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
+               <p><span class="STYLE3">(Figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
                <p>&nbsp;</p>
                <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>