Team:TU-Eindhoven/LEC/BioBricks

From 2012.igem.org

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<p>After induction for 15 hours, the cells were harvested and lysed with 5ml BugBuster containing 1µl/ml Benzonase. The supernatant of this suspension was put on a Ni-NTA column for purification and afterwards put on a PD10 column and washed with a TRIS buffer containing NaCl to remove all the calcium molecules. Then, an SDS-gel was prepared to check the purity of the GECO proteins (See figure below).</p>
<p>After induction for 15 hours, the cells were harvested and lysed with 5ml BugBuster containing 1µl/ml Benzonase. The supernatant of this suspension was put on a Ni-NTA column for purification and afterwards put on a PD10 column and washed with a TRIS buffer containing NaCl to remove all the calcium molecules. Then, an SDS-gel was prepared to check the purity of the GECO proteins (See figure below).</p>
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[[File:Groene GECO.jpg|200px|left]]
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[[File:Groene GECO.jpg|300px|left]]
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[[File:Rode GECO.jpg|200px|right]]
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[[File:Rode GECO.jpg|300px|right]]
<h3>Calcium titration</h3>
<h3>Calcium titration</h3>
<p></p>
<p></p>

Revision as of 18:11, 26 September 2012