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Team:NCTU Formosa
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| - | <p>Greenhouse Effect and the limitation of the fossil fuels have always been a concern for people on Earth. | + | <p>Greenhouse Effect and the limitation of the fossil fuels have always been a concern for people on Earth. Our team, NCTU_Formosa, constructed the synthetic pathways in E. coli to achieved high-yield, high-specificity production of isobutanol as biofuels. The <b><i>Ecofuel E.coline</i></b> project provides several optimization methods to improve the production rate of butanol as follows: (1) A suitable medium was selected. (2) Five E. coli strains were tested for isobutanol production rate. (3)Temperature-controlled RBS was used to control the expression levels of a series of metabolic proteins at the precise times by culture temperature shifts. (4) The fusion protein of a target enzyme and a zinc finger protein was constructed to increase the specificity of chemical reaction. The experimental data reveals that the <i>E.coli</i> strain DH5α grows in M9TY medium for 24 hours at 42℃have highest production of isobutanol (~0.75%). <b><i>E.coline</i></b> can provide more affordable and higher quantity of biofuel, furthermore lower the burden of our Earth. Moreover, the biosynthesis design methods can be implemented to control the expression of a series of metabolic proteins required for the maximal synthesis of a drug or energetic compound.</p></div> |
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Revision as of 18:57, 26 September 2012
Abstract
Greenhouse Effect and the limitation of the fossil fuels have always been a concern for people on Earth. Our team, NCTU_Formosa, constructed the synthetic pathways in E. coli to achieved high-yield, high-specificity production of isobutanol as biofuels. The Ecofuel E.coline project provides several optimization methods to improve the production rate of butanol as follows: (1) A suitable medium was selected. (2) Five E. coli strains were tested for isobutanol production rate. (3)Temperature-controlled RBS was used to control the expression levels of a series of metabolic proteins at the precise times by culture temperature shifts. (4) The fusion protein of a target enzyme and a zinc finger protein was constructed to increase the specificity of chemical reaction. The experimental data reveals that the E.coli strain DH5α grows in M9TY medium for 24 hours at 42℃have highest production of isobutanol (~0.75%). E.coline can provide more affordable and higher quantity of biofuel, furthermore lower the burden of our Earth. Moreover, the biosynthesis design methods can be implemented to control the expression of a series of metabolic proteins required for the maximal synthesis of a drug or energetic compound.
News
25 Sep
In order to make sure our tolerance data, we used totally three days to do this experiment. Also we collect the concentrate of isobutal in our test experiment every 4 hours in a day.And we are continuing update our wiki and part wiki.
19 Sep
This week, we try to assemble our main parts together and incubate bacteria to test the productivity of isobutanol. Team work tops everything during our cooperation. So, to finish our wiki, each of our teammates are taking charge of different parts of wiki edition, and we took turn to collect tubes for 24 hours in the isobutanol tolerance test. Except for working in the wet lab, we also had fun times taking group photos and new films! By the way, a reporter wrote a special column for us about iGem & Synthesis Biology on Sep. 17. We're now processing the information. Please stay tune and check it out then!
14 Sep
For the experiment, there are new works in for everyone. We have to measure the O.D. on 42 degree and 30 degree, test all the different system we have. The most important is that we should find the G3 part which was made last year, insert into our recent part, and measure the yield. For the wiki, all the deadline of very page sould complete by 9/24. Last, we set up a new group for devising the poster.
