Team:TU-Eindhoven/LEC/Lab
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{{:Team:TU-Eindhoven/Templates/header}} | {{:Team:TU-Eindhoven/Templates/header}} | ||
{{:Team:TU-Eindhoven/Templates/head|image=https://static.igem.org/mediawiki/2012/9/9f/Labresults.jpg}} | {{:Team:TU-Eindhoven/Templates/head|image=https://static.igem.org/mediawiki/2012/9/9f/Labresults.jpg}} | ||
+ | |||
<h3>Expected very soon!</h3> | <h3>Expected very soon!</h3> | ||
- | [[File:GECO_in_BL21.jpg|300px|link=]] | + | <h3>Plasmid construction</h3> |
+ | |||
+ | [[File:Plasmid_box.jpg|right|300px|link=]] | ||
+ | |||
+ | <p> | ||
+ | The following plasmids have been constructed: | ||
+ | <ul> | ||
+ | <li>pYES2/R-GECO, pYES2/G-GECO , pYES2/B-GECO</li> | ||
+ | <li>pYES3/R-GECO, pYES3/G-GECO , pYES3/B-GECO</li> | ||
+ | <li>pET<sub>28a</sub>/R-GECO, pET<sub>28a</sub>/G-GECO , pET<sub>28a</sub>/B-GECO</li> | ||
+ | <li>pSB1C3/R-GECO, pSB1C3/G-GECO, pSB1C3/B-GECO</li> | ||
+ | </ul> | ||
+ | |||
+ | The following plasmids were received from addgene.org: | ||
+ | <ul> | ||
+ | <li>CMV/R-GECO, CMV/G-GECO , CMV/B-GECO </li> | ||
+ | </ul> | ||
+ | |||
+ | <ul> | ||
+ | These plasmids were kindly donated by H. Iida & K. Iida: | ||
+ | <ul> | ||
+ | <li>pBCT/CCH1H, pBCT/CCH1H-HA4, pBCT/CCH1H-EGFP, YCpT-MID1, YCpT-MID1-EGFP</li> | ||
+ | </ul> | ||
+ | </p> | ||
+ | |||
+ | <h3>GECO protein expression and isolation</h3> | ||
+ | |||
+ | [[File:GECO_in_BL21.jpg|right|300px|link=]] | ||
+ | |||
+ | <p>The three GECOs have been expressed in E. coli BL21 to yield a large amount of the proteins for characterization. Cells were cultured in large Erlenmeyer flasks. The results were visually impressive: After lysis the flasks turned vibrantly red and green respectively. The blue GECO however looked much the same as the green GECO.</p> | ||
+ | |||
+ | <p><b>TODO: COLUMN FILTRATION</b></p> | ||
+ | |||
+ | <h3>Yeast transformation</h3> | ||
+ | |||
+ | [[File:Yeast_transformation.jpg|left|300px|link=]] | ||
+ | |||
+ | [[File:Transformation chart.png|right|300px|link=]] | ||
+ | |||
+ | <h3>Device tests</h3> | ||
+ | [[File:Device test.jpg|right|300px|link=]] | ||
+ | |||
+ | <h3>Spectrophotometry</h3> | ||
+ | [[File:Spectra yeast.png|right|300px|link=]] | ||
- | + | <h3></h3> | |
- | + | <h3>References</h3> | |
+ | <html> | ||
+ | <ul> | ||
+ | <li></li> | ||
+ | </ul> | ||
+ | </html> | ||
{{:Team:TU-Eindhoven/Templates/footer}} | {{:Team:TU-Eindhoven/Templates/footer}} |
Revision as of 15:06, 26 September 2012
Expected very soon!
Plasmid construction
The following plasmids have been constructed:
- pYES2/R-GECO, pYES2/G-GECO , pYES2/B-GECO
- pYES3/R-GECO, pYES3/G-GECO , pYES3/B-GECO
- pET28a/R-GECO, pET28a/G-GECO , pET28a/B-GECO
- pSB1C3/R-GECO, pSB1C3/G-GECO, pSB1C3/B-GECO
The following plasmids were received from addgene.org:
- CMV/R-GECO, CMV/G-GECO , CMV/B-GECO
-
These plasmids were kindly donated by H. Iida & K. Iida:
- pBCT/CCH1H, pBCT/CCH1H-HA4, pBCT/CCH1H-EGFP, YCpT-MID1, YCpT-MID1-EGFP
</p>
GECO protein expression and isolation
<p>The three GECOs have been expressed in E. coli BL21 to yield a large amount of the proteins for characterization. Cells were cultured in large Erlenmeyer flasks. The results were visually impressive: After lysis the flasks turned vibrantly red and green respectively. The blue GECO however looked much the same as the green GECO.</p>
<p>TODO: COLUMN FILTRATION</p>
Yeast transformation
Device tests
Spectrophotometry
References