Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

(Difference between revisions)
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|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||16.07.12
|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'||16.07.12
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|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12
+
|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||52 bp||50.0 %||87.6 °C
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|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||20.07.12
+
|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'||72 bp||48.8 %||89.8 °C
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|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'||20.07.12
+
|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'||59 bp||45.7 %||85.5 °C
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|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'||20.07.12
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|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'||41 bp||58.5 %||87.6 °C
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|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'||20.07.12
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|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'||41 bp||56.0 %||84.2 °C
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|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'||20.07.12
+
|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'||43 bp||62.7 %||90.3 °C
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|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'||20.07.12
+
|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'||43 bp||51.1 %||80.5 °C
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|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'||10.08.2012
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|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'||28 bp||53.5 %||77.0 °C
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|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'||10.08.2012
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|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'||29 bp||55.1 %||75.3 °C
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|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA-3'||10.08.2012
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|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA-3'||20 bp||50.0 %||63.5 °C
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|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC-3'||10.08.2012
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|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC-3'||20 bp||50 %||63.1 °C
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|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA-3'||20.08.2012
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|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA-3'||22 bp||59.0 %||77.2 °C
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|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG-3'||20.08.2012
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|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG-3'||26 bp||38.4 %||67.6 °C
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|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG-3'||20.08.2012
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|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG-3'||20 bp||65.0 %||68.8 °C
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|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC-3'||20.08.2012
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|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC-3'||21 bp||57.1 %||72.2 °C
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|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'||20.08.2012
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|<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'||42 bp||47.6 %||84.8 °C
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|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'||20.08.2012
+
|<div id="MS27"></div>MS27||SacB_std_10_rev||5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'||40 bp||45.0 %||76.8 °C
|}
|}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}
{{:Team:Marburg_SYNMIKRO:Template:Footer}}

Revision as of 13:00, 26 September 2012

Main Page

Primer

PrimerNameSequenceLengthG/C contentTM
MS1		AmiC_ss_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'xx bpyy %zz °C
MS2		AmiC_ss_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'xx bpyy %zz °C
MS3		HU_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA-3'16.07.12
MS4		HU_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTCv16.07.12
MS4b		HU_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG-3'10.08.2012
MS5		GroEs_std_10_fwd5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA
CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG-3'		16.07.12
MS6		GroEs_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT-3'16.07.12
MS6b		GroES_std_10_rev25'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA-3'10.08.2012
MS7		GixR_std_10_fwd5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA-3'16.07.12
MS8		GixR_std_10_rev5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT-3'16.07.12
MS9		Enhancer_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC-3'16.07.12
MS10		Enhancer_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC-3'16.07.12
MS11		GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'52 bp50.0 %87.6 °C
MS12		RBS + GIN_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC
TAGATGCTGATTGGCTATGTAAGGGTATCAACA-3'		72 bp48.8 %89.8 °C
MS13		GIN_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC-3'59 bp45.7 %85.5 °C
MS14		RFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT-3'41 bp58.5 %87.6 °C
MS15		RFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA-3'41 bp56.0 %84.2 °C
MS16		CFP_std_10_fwd5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT-3'43 bp62.7 %90.3 °C
MS17		CFP_std_10_rev5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT-3'43 bp51.1 %80.5 °C
MS18		Prefix_std_10_fwd5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG-3'28 bp53.5 %77.0 °C
MS19		Suffix_std_10_rev5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA-3'29 bp55.1 %75.3 °C
MS20		VF2_std_10_fwd5'-TGCCACCTGACGTCTAAGAA-3'20 bp50.0 %63.5 °C
MS21		VR_std_10_rev5'-ATTACCGCCTTTGAGTGAGC-3'20 bp50 %63.1 °C
MS22		SB-prep-3P-15'-GCCGCTGCAGTCCGGCAAAAAA-3'22 bp59.0 %77.2 °C
MS23		SB-prep-2Ea5'-ATGAATTCCAGAAATCATCCTTAGCG-3'26 bp38.4 %67.6 °C
MS24		Suffix_std_10_fwd5'-ACTAGTAGCGGCCGCTGCAG-3'20 bp65.0 %68.8 °C
MS25		Prefix_std_10_rev5'-TCTAGATGCGGCCGCGAATTC-3'21 bp57.1 %72.2 °C
MS26		SacB_std_10_fwd5'-CGAATTCGCGGCCGCTTCTAGATGAACATCAAAAAGTTTGC-3'42 bp47.6 %84.8 °C
MS27		SacB_std_10_rev5'-GGCTGCAGCGGCCGCTACTAGTATTATTATTTGTTAACTG-3'40 bp45.0 %76.8 °C


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