Team:Marburg SYNMIKRO/Primer

From 2012.igem.org

(Difference between revisions)

Revision as of 12:31, 26 September 2012

Primer

Primer	Name	Sequence	Length	G/C content	TM
MS1	AmiC_ss_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGTCAGGATCCAACA-3'	xx bp	yy %	zz °C
MS2	AmiC_ss_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'	xx bp	yy %	zz °C
MS3	HU_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA	16.07.12
MS4	HU_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC	16.07.12
MS4b	HU_std_10_rev2	5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG	10.08.2012
MS5	GroEs_std_10_fwd	5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG	16.07.12
MS6	GroEs_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT	16.07.12
MS6b	GroES_std_10_rev2	5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA	10.08.2012
MS7	GixR_std_10_fwd	5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA	16.07.12
MS8	GixR_std_10_rev	5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT	16.07.12
MS9	Enhancer_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC	16.07.12
MS10	Enhancer_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC	16.07.12
MS11	GIN_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA	20.07.12
MS12	RBS + GIN_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC TAGATGCTGATTGGCTATGTAAGGGTATCAACA	20.07.12
MS13	GIN_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC	20.07.12
MS14	RFP_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT	20.07.12
MS15	RFP_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA	20.07.12
MS16	CFP_std_10_fwd	5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT	20.07.12
MS17	CFP_std_10_rev	5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT	20.07.12
MS18	Prefix_std_10_fwd	5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG	10.08.2012
MS19	Suffix_std_10_rev	5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA	10.08.2012
MS20	VF2_std_10_fwd	5'-TGCCACCTGACGTCTAAGAA	10.08.2012
MS21	VR_std_10_rev	5'-ATTACCGCCTTTGAGTGAGC	10.08.2012
MS22	SB-prep-3P-1	5'-GCCGCTGCAGTCCGGCAAAAAA	20.08.2012
MS23	SB-prep-2Ea	5'-ATGAATTCCAGAAATCATCCTTAGCG	20.08.2012
MS24	Suffix_std_10_fwd	5'-ACTAGTAGCGGCCGCTGCAG	20.08.2012
MS25	Prefix_std_10_rev	5'-TCTAGATGCGGCCGCGAATTC	20.08.2012
MS26	SacB_std_10_fwd	5'-ACTAGTAGCGGCCGCTGCAG	20.08.2012
MS27	SacB_std_10_rev	5'-TCTAGATGCGGCCGCGAATTC	20.08.2012

@@ Line 77: / Line 77: @@
 |<div id="MS2"></div>MS2||AmiC_ss_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTAGCCAGACTGACCTGA-3'||xx bp||yy %||zz °C
 |-
-|<div id="MS3"></div>MS3||MS3 HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
+|<div id="MS3"></div>MS3||HU_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGAACAAGACTCAA||16.07.12
 |-
-|<div id="MS4"></div>MS4||MS4 HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
+|<div id="MS4"></div>MS4||HU_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTCTTAACTGCGTCTTTC||16.07.12
 |-
-|<div id="MS4b"></div>MS4b||MS4 (b) HU_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
+|<div id="MS4b"></div>MS4b||HU_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCTTAACTGCGTCTTTCAGTG||10.08.2012
 |-
-|<div id="MS5"></div>MS5||MS5 GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
+|<div id="MS5"></div>MS5||GroEs_std_10_fwd||5'-GCTTCTAGATGAATATTCGTCCATTGCATGATCGCGTGATCGTCAAGCGTAAAGAAGTTGAAA<br/>CTAAATCTGCTGGCGGCATCGTTCTGACCGGCTCTGCGGCGGCTAAATCCACCCGCG||16.07.12
 |-
-|<div id="MS6"></div>MS6||MS6 GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
+|<div id="MS6"></div>MS6||GroEs_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTACGCTTCAACAAT||16.07.12
 |-
-|<div id="MS6b"></div>MS6b||MS6 (b) GroES_sTd_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
+|<div id="MS6b"></div>MS6b||GroES_std_10_rev2||5'-GCCTGCAGCGGCCGCTACTAGTACCCGCTTCAACAATTGCCAGAA||10.08.2012
 |-
-|<div id="MS7"></div>MS7||MS7 GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
+|<div id="MS7"></div>MS7||GixR_std_10_fwd||5'-CTAGAGCGTTATCCAAAACCTCGGTTTACAGGAATACTAGTAGCGGCCGCTGCA||16.07.12
 |-
-|<div id="MS8"></div>MS8||MS8 GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
+|<div id="MS8"></div>MS8||GixR_std_10_rev||5'-GCGGCCGCTACTAGTATTCCTGTAAACCGAGGTTTTGGATAACGCT||16.07.12
 |-
-|<div id="MS9"></div>MS9||MS9 Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
+|<div id="MS9"></div>MS9||Enhancer_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGGGGTATCAACAAATGACC||16.07.12
 |-
-|<div id="MS10"></div>MS10||MS10 Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
+|<div id="MS10"></div>MS10||Enhancer_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTTGTTCACATCCTGCAC||16.07.12
 |-
-|<div id="MS11"></div>MS11||MS11 GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+|<div id="MS11"></div>MS11||GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
 |-
-|<div id="MS12"></div>MS12||MS12 RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
+|<div id="MS12"></div>MS12||RBS + GIN_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGAAAGAGGGGACAAAC<br/>TAGATGCTGATTGGCTATGTAAGGGTATCAACA||20.07.12
 |-
-|<div id="MS13"></div>MS13||MS13 GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
+|<div id="MS13"></div>MS13||GIN_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAATTGATTCGATCGTCGTTTTCTATATGCGC||20.07.12
 |-
-|<div id="MS14"></div>MS14||MS14 RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
+|<div id="MS14"></div>MS14||RFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGCTTCCTCCGAAGACGTT||20.07.12
 |-
-|<div id="MS15"></div>MS15||MS15 RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
+|<div id="MS15"></div>MS15||RFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTAAGCACCGGTGGA||20.07.12
 |-
-|<div id="MS16"></div>MS16||MS16 CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
+|<div id="MS16"></div>MS16||CFP_std_10_fwd||5'-GCGAATTCGCGGCCGCTTCTAGAGTGAGCAAGGGCGAGGAGCT||20.07.12
 |-
-|<div id="MS17"></div>MS17||MS17 CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
+|<div id="MS17"></div>MS17||CFP_std_10_rev||5'-GCCTGCAGCGGCCGCTACTAGTATTATTACTTGTACAGCTCGT||20.07.12
 |-
-|<div id="MS18"></div>MS18||MS 18 Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
+|<div id="MS18"></div>MS18||Prefix_std_10_fwd||5'-GTTTCTTCGAATTCGCGGCCGCTTCTAG||10.08.2012
 |-
-|<div id="MS19"></div>MS19||MS 19 Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
+|<div id="MS19"></div>MS19||Suffix_std_10_rev||5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTA||10.08.2012
 |-
-|<div id="MS20"></div>MS20||MS 20 VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
+|<div id="MS20"></div>MS20||VF2_std_10_fwd||5'-TGCCACCTGACGTCTAAGAA||10.08.2012
 |-
-|<div id="MS21"></div>MS21||MS 21 VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
+|<div id="MS21"></div>MS21||VR_std_10_rev||5'-ATTACCGCCTTTGAGTGAGC||10.08.2012
 |-
-|<div id="MS22"></div>MS22||MS22 SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
+|<div id="MS22"></div>MS22||SB-prep-3P-1||5'-GCCGCTGCAGTCCGGCAAAAAA||20.08.2012
 |-
-|<div id="MS23"></div>MS23||MS23 SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
+|<div id="MS23"></div>MS23||SB-prep-2Ea||5'-ATGAATTCCAGAAATCATCCTTAGCG||20.08.2012
 |-
-|<div id="MS24"></div>MS24||MS24 Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
+|<div id="MS24"></div>MS24||Suffix_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012
 |-
-|<div id="MS25"></div>MS25||MS25 Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
+|<div id="MS25"></div>MS25||Prefix_std_10_rev||5'-TCTAGATGCGGCCGCGAATTC||20.08.2012
 |-
 |<div id="MS26"></div>MS26||SacB_std_10_fwd||5'-ACTAGTAGCGGCCGCTGCAG||20.08.2012