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Team:Tuebingen/Results
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== Identified Problems == | == Identified Problems == | ||
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| + | * '''PCR problems''': Some PCRs yielded only products in very low concentrations. Some primers also amplified multiple fragments. New primers partially solved these troubles. | ||
| + | * | ||
== Progress Illustraion == | == Progress Illustraion == | ||
The following illustraion gives a quick overview of the final status of our lab work. | The following illustraion gives a quick overview of the final status of our lab work. | ||
[[File:Tue-labmap-results.png|600px|thumb|center|final status of our parts]] | [[File:Tue-labmap-results.png|600px|thumb|center|final status of our parts]] | ||
Revision as of 10:27, 26 September 2012
Results
Contents |
Parts Statuses
- Pfet3, Panb1, lacZ: PCR was not successful for many tries. For Pfet3, new primers were designed but there was no time left to complete this part.
- Tadh1: Tadh1 has been successfully amplified and ligation in pGEM vectors is under way.
- pRS313, pRS315, pRS316: The shuttle plasmids are available and digested.
- Padh1, Psuc2, rox1, mig1, luciferase, mPR Danio rerio, mPR Xenopus laevis: These parts are all available and digested. The ligation in both pSB1C3 and our pRS shuttle plasmids was so far not successful.
Identified Problems
- PCR problems: Some PCRs yielded only products in very low concentrations. Some primers also amplified multiple fragments. New primers partially solved these troubles.
Progress Illustraion
The following illustraion gives a quick overview of the final status of our lab work.
