Team:TU-Eindhoven/LEC/BioBricks

From 2012.igem.org

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<h3>Harvesting and Purification</h3>
<h3>Harvesting and Purification</h3>
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<p>After induction for 15 hours, the cells were harvested and lysed with 5ml BugBuster containing 1µl/ml Benzonase. The supernatant of this suspension was put on a Ni-NTA column for purification and afterwards put on a PD10 column and washed with a TRIS buffer containing NaCl to remove all the calcium molecules. Then, an SDS-gel was prepared to check the purity of the GECO proteins (See figure below).  
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<p>After induction for 15 hours, the cells were harvested and lysed with 5ml BugBuster containing 1µl/ml Benzonase. The supernatant of this suspension was put on a Ni-NTA column for purification and afterwards put on a PD10 column and washed with a TRIS buffer containing NaCl to remove all the calcium molecules. Then, an SDS-gel was prepared to check the purity of the GECO proteins (See figure below).</p>
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[[File:SDS-gel.jpg|500px|left]]</p>
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[[File:SDS-gel.jpg|500px|left]]
<h3>Calcium titration</h3>
<h3>Calcium titration</h3>
<p></p>
<p></p>

Revision as of 09:37, 26 September 2012