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Team:Exeter/lab book/gibs/wk6
From 2012.igem.org
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<!<p><b>**Tuesday 14.8.12**</b></p><br><p> | <!<p><b>**Tuesday 14.8.12**</b></p><br><p> | ||
| - | </p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation </u></a>of <i>wcfA</i>-term (BBa_K764010)</p> | + | </p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation </u></a>of <i>wcfA</i>-term (BBa_K764010)</p><br> |
Revision as of 17:28, 25 September 2012
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Operon Construction: 13th - 17th August 2012 **Tuesday 14.8.12**Transformation of wcfA-term (BBa_K764010) **Wednesday 15.8.12** Transferred wcfA-term colonies to LB(tet) medium **Thursday 16.8.12** wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C Supernatant discarded Step 1045µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation Nanodropped for following results: (A) 329.7 ng/µl (B) 50.0 ng/µl (C) 206.6 ng/µl Made streak plates from culture dregs |
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