"
Team:Evry/Data
From 2012.igem.org
(Difference between revisions)
| Line 28: | Line 28: | ||
<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/File:Degron_part2.jpg" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/c/c4/Degron_part2.jpg" width="500" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="/File:Degron_part2.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.</div></div></div></div> | <div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:502px;"><a href="/File:Degron_part2.jpg" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/c/c4/Degron_part2.jpg" width="500" class="thumbimage" /></a> <div class="thumbcaption"><div class="magnify"><a href="/File:Degron_part2.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.</div></div></div></div> | ||
| - | <p> | + | <p> |
| + | <ul> | ||
| + | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">K812010:</a>GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system | ||
| + | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">K812012:</a>OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin | ||
| + | <li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812013">K812013:</a></p>The Auxin reciever system assembled by golden gate method composed of GFP-AID OsTirI polysistronic system for auxin detection in tadpole</ul> | ||
| + | </p> | ||
<p>Which ones are characterized and result in one sentence</p> | <p>Which ones are characterized and result in one sentence</p> | ||
Revision as of 23:32, 24 September 2012
Data page: Summary of what we have done this year (so far!)
New frog plasmids
- K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system
One characterization image
One plasmid map
The auxin system
The auxin receiver system
- K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
- K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
- K812013:The Auxin reciever system assembled by golden gate method composed of GFP-AID OsTirI polysistronic system for auxin detection in tadpole
Which ones are characterized and result in one sentence
The auxin emission system
Goldenbricks
Summary
The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.
Our favourites parts
- K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
- K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
The other parts we have created
- K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
- K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
- K812053: A GoldenBricked version of the strong RBS B0034
- K812054: A GoldenBricked version of the RFP E1010
- K812055: A GoldenBricked version of the terminator B0015
- K812056: A GoldenBricked version of the pLac R0010 promoter
- K812057: A GoldenBricked of an sfGFP protein
- K812058: A GoldenBricked of medium strenght RBS J61107
- K812059: A GoldenBricked of week RBS J61117
say we are testing them
