Team:Evry/Data

From 2012.igem.org

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<h1>New frog plasmids</h1>
<h1>New frog plasmids</h1>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:302px;"><a href="/File:PSC2_plasmid.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/9/90/PSC2_plasmid.png" width="300" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:PSC2_plasmid.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.</div></div></div></div>
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<div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:302px;"><a href="/File:PSC2_plasmid.png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2012/9/90/PSC2_plasmid.png" width="300" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/File:PSC2_plasmid.png" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>Fig 3: a. Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.</div></div></div></div>
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<p>Name of the parts, and link to the registry</p>
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<p>
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<ul>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812000">K812000:</a> PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system
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</ul>
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</p>
<p>One characterization image</p>
<p>One characterization image</p>

Revision as of 21:43, 24 September 2012

Data page: Summary of what we have done this year (so far!)



New frog plasmids

Fig 3: a. Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

  • K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

One characterization image

One plasmid map

The auxin system

The auxin receiver system

Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.

List of the parts with links to the registry

Which ones are characterized and result in one sentence

The auxin emission system

Fig 5: a. The vector, promoter, and terminator are in excess: the assembly produces mostly monosistrons. b. The protein sequences and RBS are in excess: the assembly produces mostly polysistrons.
List of the parts with links to the registry Which ones are characterized and result in one sentence

Goldenbricks

Summary

Fig 4: Summary of the GoldenBrick procedure

The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

Our favourites parts

  • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
  • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

The other parts we have created

  • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
  • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
  • K812053: A GoldenBricked version of the strong RBS B0034
  • K812054: A GoldenBricked version of the RFP E1010
  • K812055: A GoldenBricked version of the terminator B0015
  • K812056: A GoldenBricked version of the pLac R0010 promoter
  • K812057: A GoldenBricked of an sfGFP protein
  • K812058: A GoldenBricked of medium strenght RBS J61107
  • K812059: A GoldenBricked of week RBS J61117

say we are testing them

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