"
Team:TU Darmstadt/Protocols/pNP Assay
From 2012.igem.org
(Difference between revisions)
(→pNP-Assay) |
(→About) |
||
| Line 41: | Line 41: | ||
== pNP-Assay == | == pNP-Assay == | ||
=== About === | === About === | ||
| - | + | pNP-assays are a common way to quantify hydrolytic activity. We use ''para-Nitrophenylbutyrate'' (pNPB) as a substrate. As the catalysts we use the enzymes[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808025 FsC], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808026 pNB-Est13] or our transformed and induced bacteria. | |
| + | |||
| + | Conditions: T = 34°C pH = 7.4 | ||
| + | |||
| + | Method: Measurement of absorption at 405nm every minute over 30 minutes, after addition of enzyme | ||
| + | |||
| + | [[File:pnpb_spalt.png]] | ||
=== Enzyme === | === Enzyme === | ||
=== Bacteria === | === Bacteria === | ||
Revision as of 17:48, 24 September 2012
Contents |
pNP-Assay
About
pNP-assays are a common way to quantify hydrolytic activity. We use para-Nitrophenylbutyrate (pNPB) as a substrate. As the catalysts we use the enzymes[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808025 FsC], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K808026 pNB-Est13] or our transformed and induced bacteria.
Conditions: T = 34°C pH = 7.4
Method: Measurement of absorption at 405nm every minute over 30 minutes, after addition of enzyme
