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Team:Macquarie Australia/Protocols/SDSPAGE
From 2012.igem.org
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<center><h1>SDS-PAGE</h1></center> | <center><h1>SDS-PAGE</h1></center> | ||
| - | <center>Pelleted cells were resuspended in 200 uL Milli-Q H2O. <br> | + | <center>Pelleted cells were resuspended in 200 uL Milli-Q H2O. </center> <br> |
<center>Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. </center><br> | <center>Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. </center><br> | ||
<center>Using a Hamilton syringe, the cells were sheared. </center><br> | <center>Using a Hamilton syringe, the cells were sheared. </center><br> | ||
<center>Centrifuged the preparations for 3 mins @ 13,000 rpm.</center> <br> | <center>Centrifuged the preparations for 3 mins @ 13,000 rpm.</center> <br> | ||
<center>Loaded 20 uL of the supernatant in to the gel. </center> <br> | <center>Loaded 20 uL of the supernatant in to the gel. </center> <br> | ||
Revision as of 12:24, 24 September 2012
