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Team:Macquarie Australia/Protocols/SDSPAGE
From 2012.igem.org
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{{:Team:Macquarie_Australia/Template/MQ12}} | {{:Team:Macquarie_Australia/Template/MQ12}} | ||
<center><h1>SDS-PAGE</h1></center> | <center><h1>SDS-PAGE</h1></center> | ||
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| + | Pelleted cells were resuspended in 200 uL Milli-Q H2O. | ||
| + | Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. | ||
| + | Using a Hamilton syringe, the cells were sheared. | ||
| + | Centrifuged the preparations for 3 mins @ 13,000 rpm. | ||
| + | Loaded 20 uL of the supernatant in to the gel. | ||
Revision as of 12:22, 24 September 2012
SDS-PAGE
Pelleted cells were resuspended in 200 uL Milli-Q H2O. Transferred 50 uL of suspension into new Eppendorf tubes and combined with 50 uL 2x TruSep sample buffer. Using a Hamilton syringe, the cells were sheared. Centrifuged the preparations for 3 mins @ 13,000 rpm. Loaded 20 uL of the supernatant in to the gel.
