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Team:Wageningen UR/Journal/week21
From 2012.igem.org
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| + | ''17 September'' | ||
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| + | * ligation of the PCR products from 14 September (mixing the duplo samples together) with pJET and transformation with JM109 | ||
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| + | ''18 September'' | ||
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| + | * colony PCR of the transformation from 17 September | ||
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week19 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week21 next week]] | [[https://2012.igem.org/Team:Wageningen_UR/Journal/week19 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week21 next week]] | ||
Revision as of 18:08, 23 September 2012
week 21: 17 September - 23 September
Hepatitis B inside modification
- Colony PCR of Hepatitis B core protein behing an IPTG promoter in pSB1C3 backbone with sequencing primers
-> the gel shows fragments of the expected size (about 1kb)
GFP modification
17 September
- ligation of the PCR products from 14 September (mixing the duplo samples together) with pJET and transformation with JM109
18 September
- colony PCR of the transformation from 17 September
