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Revision as of 16:09, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) ← Older edit		Revision as of 16:21, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) Newer edit →
Line 21:		Line 21:
	-yeast		-yeast
	assemble plasmids		assemble plasmids
-	His tag plasmids	+	integrative - industrial
		+	centromeric - Ethan
		+	episomal - Guy
		+	His tag plasmids - Dave
	n-terminus		n-terminus
	c-terminus		c-terminus
-	clone mating factor-alpha	+	clone mating factor-alpha Steven
	-bacteria		-bacteria
-	assemble plasmids	+	assemble plasmids (3A) - Steven
-	His tag plasmids	+	His tag plasmids - Dave
	n-terminus		n-terminus
	c-terminus		c-terminus
-	secretion	+	secretion -Ethan
-	-Prolyl Endoprotease-A.niger	+	-Prolyl Endoprotease-A.niger Guy
	viable option?		viable option?
	source?  cloning vs synthesize		source?  cloning vs synthesize
Line 37:		Line 40:
	yeast		yeast
	-functional protease assay		-functional protease assay
-	fluorophore/quencher	+	fluorophore/quencher - Ryan
-	mass spec	+	mass spec - Ryan/Guy
-	elisa	+	elisa - Ryan/Guy
		+	-Modeling - Dave
	== Results ==		== Results ==

---

Revision as of 16:21, 19 June 2012

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)

Project Details

-yeast

 assemble plasmids
   integrative - industrial
   centromeric - Ethan
   episomal - Guy
 His tag plasmids - Dave
  n-terminus
  c-terminus
 clone mating factor-alpha Steven

-bacteria

 assemble plasmids (3A) - Steven
 His tag plasmids - Dave
  n-terminus
  c-terminus
 secretion -Ethan

-Prolyl Endoprotease-A.niger Guy

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher - Ryan
 mass spec - Ryan/Guy
 elisa - Ryan/Guy

-Modeling - Dave

Results