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Revision as of 16:06, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) ← Older edit		Revision as of 16:07, 19 June 2012 (view source) Btsearcy (Talk | contribs) (→Project Details) Newer edit →
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	== Project Details==		== Project Details==
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-	IGEM
-	-PCR supplies
-	-order peptide/quencher
	-yeast		-yeast
	assemble plasmids		assemble plasmids

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Revision as of 16:07, 19 June 2012

Home	Team	Official Team Profile	Project	Parts Submitted to the Registry	Modeling	Notebook	Safety	Attributions

Overall project

We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)

Project Details

-yeast

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus
 clone mating factor-alpha

-bacteria

 assemble plasmids
 His tag plasmids
  n-terminus
  c-terminus

-Prolyl Endoprotease-A.niger

 viable option?
 source?  cloning vs synthesize
   E.coli
   yeast

-functional protease assay

 fluorophore/quencher
 mass spec
 elisa

Results