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Team:TU-Eindhoven/LEC/LabTheory
From 2012.igem.org
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| - | + | '''Yeast versus E. Coli''' | |
<p>Before this light emitting cell display project could start, it was necessary to decide whether E. Coli or yeast would be used. As both competent cell types are able to develop rapidly, take care of protein expression and are cheap to culture, the decision was made upon the difference in complexity of the cell types. Not only with a few native calcium channels, which exist in both cell types, our iGEM team would be able to reach the goal of this project, but also overexpression of voltage-gated calcium channel was needed. As soon as it was found that CCH1-MID1, a homologous to mammalian voltage-gated calcium channels, could be overexpressed in S. Cerevisiae and facilitates the influx of calcium upon electrical stimuli, it was easily decided to use yeast as competent cells for our project.</p> | <p>Before this light emitting cell display project could start, it was necessary to decide whether E. Coli or yeast would be used. As both competent cell types are able to develop rapidly, take care of protein expression and are cheap to culture, the decision was made upon the difference in complexity of the cell types. Not only with a few native calcium channels, which exist in both cell types, our iGEM team would be able to reach the goal of this project, but also overexpression of voltage-gated calcium channel was needed. As soon as it was found that CCH1-MID1, a homologous to mammalian voltage-gated calcium channels, could be overexpressed in S. Cerevisiae and facilitates the influx of calcium upon electrical stimuli, it was easily decided to use yeast as competent cells for our project.</p> | ||
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Revision as of 14:07, 23 September 2012
Before this light emitting cell display project could start, it was necessary to decide whether E. Coli or yeast would be used. As both competent cell types are able to develop rapidly, take care of protein expression and are cheap to culture, the decision was made upon the difference in complexity of the cell types. Not only with a few native calcium channels, which exist in both cell types, our iGEM team would be able to reach the goal of this project, but also overexpression of voltage-gated calcium channel was needed. As soon as it was found that CCH1-MID1, a homologous to mammalian voltage-gated calcium channels, could be overexpressed in S. Cerevisiae and facilitates the influx of calcium upon electrical stimuli, it was easily decided to use yeast as competent cells for our project.
