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Team:TU Munich/Project/Genome Integration
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As we can't obey the letters of the German Purity Law (there is a zero tolerance policy concerning transgenic ingredients), we try our best to meet the spirit of the law. Thus, it is '''unacceptable for us to work with antibiotics''' to keep up the selective environment. Since we can't work with '''auxotrophies in beer''' either, we have to make sure the yeasts don't get rid of the biobricks. The most promising way to accomplish a long lasting presence of our constructs is to achieve '''genome integration'''. | As we can't obey the letters of the German Purity Law (there is a zero tolerance policy concerning transgenic ingredients), we try our best to meet the spirit of the law. Thus, it is '''unacceptable for us to work with antibiotics''' to keep up the selective environment. Since we can't work with '''auxotrophies in beer''' either, we have to make sure the yeasts don't get rid of the biobricks. The most promising way to accomplish a long lasting presence of our constructs is to achieve '''genome integration'''. | ||
| - | By means of genome integration we don't have to induce a selective stress in the brewing process and yet can be sure that the yeasts express our constructs properly - under constitutive promoters like TEF1 or TEF2 as well as our own [[Ethanol Inducible | + | By means of genome integration we don't have to induce a selective stress in the brewing process and yet can be sure that the yeasts express our constructs properly - under constitutive promoters like TEF1 or TEF2 as well as our own [[Ethanol Inducible Promoter]] or the [[Light Switchable Promoter]]. |
* Necessity for Genome integration as no auxotrophies/resistances can be added to the beer | * Necessity for Genome integration as no auxotrophies/resistances can be added to the beer | ||
Revision as of 16:08, 22 September 2012
Contents |
Genome Integration
As we can't obey the letters of the German Purity Law (there is a zero tolerance policy concerning transgenic ingredients), we try our best to meet the spirit of the law. Thus, it is unacceptable for us to work with antibiotics to keep up the selective environment. Since we can't work with auxotrophies in beer either, we have to make sure the yeasts don't get rid of the biobricks. The most promising way to accomplish a long lasting presence of our constructs is to achieve genome integration.
By means of genome integration we don't have to induce a selective stress in the brewing process and yet can be sure that the yeasts express our constructs properly - under constitutive promoters like TEF1 or TEF2 as well as our own Ethanol Inducible Promoter or the Light Switchable Promoter.
- Necessity for Genome integration as no auxotrophies/resistances can be added to the beer
- Increased of expression
Background and principles
- Available BioBrick
- Short description of the principle
Idea
- Plan to integrate mOrange, Thaumatin and Limonene and Brew beer with it
