Team:SUSTC-Shenzhen-B/week 3

From 2012.igem.org

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                 <h2>September</h2>
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                 <h2>July</h2>
                 <h3>Abstract</h3>
                 <h3>Abstract</h3>
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                 <p>This is the last week and our work almost comes to the end. Our team members spares no efforts to finish their own tasks. </p>
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                 <p>This week we plan to have a short vacation and discuss the next step of our project.</p>
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                <h3>Week 3</h3>
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                <h3>7.29.2012-7.31.2012</h3>
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<p>7.29.2012</p>
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<p>We took a break to get ready for the week ahead of us.</p>
<br>
<br>
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                <h3>Week 2</h3>
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<p>7.30.2012</p>
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                <h3>9.16.2012-9.22.2012</h3>
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<p>We moved our work place from school to BGI to do some experiment there. All of us were excited about the move.</p>
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<p>9.17.2012</p>
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<p>After all the material we bought arrived, we started our lab work again in our school. It’s a hard work to develop our own laboratory. But we had fun at the same time.</p>
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<p>We did a digest and amplify GFP & RFP with PCR.
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</p>
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<br>
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<p>9.18.2012-9.20.2012</p>
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<p>7.31.2012</p>
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<p>We visited 3 high schools and gave presentations to their students on synthetic biology and our IGEM projects. There are many students being interested in biology and some of them would like to join this competition. We think our major goal has been achieved.
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<p>1. We divided our team members into 2 groups. One will be focusing on reading paper and looking for the right terminator; the other one will be focusing on designing and doing experiment.</p>
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We also did a ligation to connect GFP, RFP and the plasmid together. Then we did a transformation.
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<p>2. Our adviser Chen Yu gave us an instruction session an molecular biology to help us get started our work.</p>
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</p>
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<p>3. Moving to a new place is not an easy thing. We cleaned our dormitory. </p>
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<br>
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<p>9.21.2012</p>
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<p>Today’s morning, we saw that control group almost exist only few of bacterial colony and the experimental group grow lots of colonies. I think it is a positive suggest. So we did a colony PCR to test whether the ligation is successful. We picked up 10 colonies to test and three of them is positive ligated by three parts and all the colonies are positive ligated by two parts.</p>
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<br>
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<p>9.22.2012</p>
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<p>We picked up those colonies which are positive.  Through the bacterium culture in shake-flask more than 12 hours, we can do plasmid miniprep.</p>
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<p>We also started to record our open classes. </p>
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<p>Because the deadline of wiki is coming so almost every team members are busy in writing their parts.</p>
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                 <h2>Week Diary</h2>
                 <h2>Week Diary</h2>
                 <ul class="tmo_ul_list">
                 <ul class="tmo_ul_list">
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                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/"september>week 1</a></li>
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                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/july">week 1</a></li>
                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2">week 2</a></li>
                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2">week 2</a></li>
                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3">week 3</a></li>
                     <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3">week 3</a></li>
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                 </ul>
                 </ul>
      
      

Revision as of 00:11, 27 September 2012

SUSTC iGEM Theme - Free CSS Template

SUSTC iGEM Theme - Free CSS Template

July

Abstract

This week we plan to have a short vacation and discuss the next step of our project.

Week 3

7.29.2012-7.31.2012

7.29.2012

We took a break to get ready for the week ahead of us.


7.30.2012

We moved our work place from school to BGI to do some experiment there. All of us were excited about the move.


7.31.2012

1. We divided our team members into 2 groups. One will be focusing on reading paper and looking for the right terminator; the other one will be focusing on designing and doing experiment.

2. Our adviser Chen Yu gave us an instruction session an molecular biology to help us get started our work.

3. Moving to a new place is not an easy thing. We cleaned our dormitory.

Week Diary