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Team:Osaka/week8
From 2012.igem.org
(Difference between revisions)
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# Ligation | # Ligation | ||
#* 167(C):1-1D(upstream)+160(downstream)+1-3A(vector) | #* 167(C):1-1D(upstream)+160(downstream)+1-3A(vector) | ||
| - | # Transformation of plasmid DNA into <i>E.coli</i> | + | # Transformation of ligation products into DH5α <i>E.coli</i> |
| + | # Transformation of plasmid DNA into <i>E.coli</i>HST04 165,166 | ||
# Preparation of glycerol stock of cell culture:161~166 | # Preparation of glycerol stock of cell culture:161~166 | ||
# Restriction digests | # Restriction digests | ||
Revision as of 13:15, 20 September 2012
September 16 (Sun)
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Gel extraction :161,162
- Ligation
- 163(C):161(upstream)+151(downstream)+1-3A(vector)
- 164(C):161(upstream)+152(downstream)+1-3A(vector)
- 165(C):162(upstream)+151(downstream)+1-3A(vector)
- 166(C):162(upstream)+152(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
- Transfer to liquid culture
- Tolerance assay(140,150)
September 17 (Mon)
- Miniprep of yesterday's culture:161,162
- Restriction digests of minipreppped parts
- Transfer to liquid culture:163~166,
- Electrophoresis of digests
- Tolerance assay(WT)
September 18 (Tue)
- Miniprep of yesterday's culture:163~166
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Transfer to liquid culture
September 19 (Wed)
- Gel extraction :161,162
- Transfer to liquid culture:161,162
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Electrophoresis of digests
- Transformation of plasmid DNA into E.coliDH5α 163~166 / HST04 161,162
September 20 (Thu)
- Miniprep (164~166)
- Restriction digests of minipreppped parts
- Electrophoresis of digests
- Tolerance assay(149,150,152,WT)
- Sequencing (160~162,165,166)
- Restriction digests(1-1D)
- Ligation
- 167(C):1-1D(upstream)+160(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
- Transformation of plasmid DNA into E.coliHST04 165,166
- Preparation of glycerol stock of cell culture:161~166
- Restriction digests
- Ligation
- 168(T):1-1D(upstream)+153(downstream)+1-7A(vector)
- 169(T):159upstream)+153(downstream)+1-7A(vector)
- 170(T):1-9N(upstream)+153(downstream)+1-7A(vector)
- 171(T):1-23L(upstream)+154(downstream)+1-7A(vector)
- 172(C):1-2D(upstream)+1-23L(downstream)+1-3A(vector)
- Transformation of ligation products into DH5α E.coli
