Team:Colorado State/Project
From 2012.igem.org
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== Project Details== | == Project Details== | ||
- | + | IGEM | |
- | + | -PCR supplies | |
- | + | -order peptide/quencher | |
- | + | -yeast | |
- | + | assemble plasmids | |
- | + | His tag plasmids | |
- | + | n-terminus | |
- | + | c-terminus | |
- | + | clone mating factor-alpha | |
- | + | -bacteria | |
- | + | assemble plasmids | |
- | + | His tag plasmids | |
- | + | n-terminus | |
- | + | c-terminus | |
- | + | -Prolyl Endoprotease-A.niger | |
- | + | viable option? | |
- | + | source? cloning vs synthesize | |
- | + | E.coli | |
- | + | yeast | |
+ | -functional protease assay | ||
+ | fluorophore/quencher | ||
+ | mass spec | ||
+ | elisa | ||
== Results == | == Results == |
Revision as of 16:06, 19 June 2012
Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
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Overall project
We are currently pursuing the concept of brewing a gluten-free beer using a yeast that secretes an enzyme to break down the gluten. (1-2 paragraphs)
Project Details
IGEM -PCR supplies -order peptide/quencher -yeast
assemble plasmids His tag plasmids n-terminus c-terminus clone mating factor-alpha
-bacteria
assemble plasmids His tag plasmids n-terminus c-terminus
-Prolyl Endoprotease-A.niger
viable option? source? cloning vs synthesize E.coli yeast
-functional protease assay
fluorophore/quencher mass spec elisa