Team:Osaka/week8

From 2012.igem.org

(Difference between revisions)
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# Restriction digests of minipreppped parts
# Restriction digests of minipreppped parts
# Gel electrophoresis of digests
# Gel electrophoresis of digests
-
# Transformation of plasmid DNA into <i>E.coli</i> HST04 161~166
 
# Transfer to liquid culture
# Transfer to liquid culture
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# Restriction digests(1-1D)
# Restriction digests(1-1D)
# Ligation  
# Ligation  
-
#* 163(C):1-1D(upstream)+160(downstream)+1-3A(vector)
+
#* 167(C):1-1D(upstream)+160(downstream)+1-3A(vector)
# Transformation of plasmid DNA into <i>E.coli</i>DH5α 167 / HST04 165,166
# Transformation of plasmid DNA into <i>E.coli</i>DH5α 167 / HST04 165,166
# Preparation of glycerol stock of cell culture:161~166
# Preparation of glycerol stock of cell culture:161~166
 +
# Restriction digests
 +
# Ligation
 +
#* 168(T):1-1D(upstream)+153(downstream)+1-7A(vector)
 +
#* 169(T):159upstream)+153(downstream)+1-7A(vector)
 +
#* 170(T):1-9N(upstream)+153(downstream)+1-7A(vector)
 +
#* 171(T):1-23L(upstream)+154(downstream)+1-7A(vector)
 +
#* 172(T):1-2D(upstream)+1-23L(downstream)+1-3A(vector)
 +
# Transformation of ligation products into DH5α <i>E.coli</i>

Revision as of 13:11, 20 September 2012


September 16 (Sun)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Gel extraction :161,162
  5. Ligation
    • 163(C):161(upstream)+151(downstream)+1-3A(vector)
    • 164(C):161(upstream)+152(downstream)+1-3A(vector)
    • 165(C):162(upstream)+151(downstream)+1-3A(vector)
    • 166(C):162(upstream)+152(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Transfer to liquid culture
  8. Tolerance assay(140,150)

September 17 (Mon)

  1. Miniprep of yesterday's culture:161,162
  2. Restriction digests of minipreppped parts
  3. Transfer to liquid culture:163~166,
  4. Electrophoresis of digests
  5. Tolerance assay(WT)

September 18 (Tue)

  1. Miniprep of yesterday's culture:163~166
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transfer to liquid culture

September 19 (Wed)

  1. Gel extraction :161,162
  2. Transfer to liquid culture:161,162
  3. Miniprep of yesterday's culture
  4. Restriction digests of minipreppped parts
  5. Electrophoresis of digests
  6. Transformation of plasmid DNA into E.coliDH5α 163~166 / HST04 161,162

September 20 (Thu)

  1. Miniprep (164~166)
  2. Restriction digests of minipreppped parts
  3. Electrophoresis of digests
  4. Tolerance assay(149,150,152,WT)
  5. Sequencing (160~162,165,166)
  6. Restriction digests(1-1D)
  7. Ligation
    • 167(C):1-1D(upstream)+160(downstream)+1-3A(vector)
  8. Transformation of plasmid DNA into E.coliDH5α 167 / HST04 165,166
  9. Preparation of glycerol stock of cell culture:161~166
  10. Restriction digests
  11. Ligation
    • 168(T):1-1D(upstream)+153(downstream)+1-7A(vector)
    • 169(T):159upstream)+153(downstream)+1-7A(vector)
    • 170(T):1-9N(upstream)+153(downstream)+1-7A(vector)
    • 171(T):1-23L(upstream)+154(downstream)+1-7A(vector)
    • 172(T):1-2D(upstream)+1-23L(downstream)+1-3A(vector)
  12. Transformation of ligation products into DH5α E.coli


September 21 (Fri)

September 22 (Sat)

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