Team:Tuebingen/NotebookAppendix
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=== Constructs === | === Constructs === | ||
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<table border="solid"> | <table border="solid"> | ||
- | <tr><th>pRS</th><th> | + | <tr><th>pRS</th><th>part #1</th><th>part #2</th><th>part #3</th></tr> |
<tr><td>313</td><td>Padh1</td><td>mPR danio rerio</td><td>Tadh1</td></tr> | <tr><td>313</td><td>Padh1</td><td>mPR danio rerio</td><td>Tadh1</td></tr> | ||
<tr><td>313</td><td>Padh1</td><td>mPR Xenopus laevis</td><td>Tadh1</td></tr> | <tr><td>313</td><td>Padh1</td><td>mPR Xenopus laevis</td><td>Tadh1</td></tr> |
Revision as of 13:55, 20 September 2012
Contents |
Appendix
To complete this report we list all products and software used over the course of our project.
Constructs
pRS | part #1 | part #2 | part #3 |
---|---|---|---|
313 | Padh1 | mPR danio rerio | Tadh1 |
313 | Padh1 | mPR Xenopus laevis | Tadh1 |
315 | Pfet3 | rox1 | Tadh1 |
315 | Pfet3 | mig1 | Tadh1 |
316 | Panb1 | lacZ | Tadh1 |
316 | Panb1 | luciferase | Tadh1 |
316 | Psuc2 | lacZ | Tadh1 |
316 | Psuc2 | luciferase | Tadh1 |
Protocols
Kits
- Genaxxon Plasmid DNA Purification Mini Prep
- Genaxxon Gel Extraction Mini Prep
- Genaxxon PCR DNA Purification Mini Prep
- Qiagen Plasmid Midi
Chemicals
We needed the following chemicals:
- Ampicillin
beta-lactam antibiotic - Agarose
Polysaccharide, major component of Agar - Dimethylsulfoxid (DMSO)
organic solvent - Acetic Acid
- Ethylenediaminetetraacetic acid (EDTA)
- Ethanol
- TRIS
buffer solution for enzymes - Nucleoside triphosphate
- Trypton
- Isopropanol
- Isopropyl-β-D-thiogalactopyranosid (IPTG)
- LB-medium
used for growth of E.coli - Agar-Agar
Software
The following list of software was used in the team:
- [http://ugene.unipro.ru/ Unipro UGENE]
UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
- [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial)
All our primers were designed using Vector NTI which is also used by our advisors.
- [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST]
BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
- [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
- [http://drive.google.com Google Drive]
Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).