Team:Exeter/lab book/gibs/wk6

From 2012.igem.org

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     <!------------ENTER LAB BOOK HERE: PLEASE INCLUDE DATES WITHIN THE WEEK------------>  
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     <!<p><b>**Tuesday 14.8.12**</b></p><br><p>
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</p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#1d1d1b"><u>Transformation </u></a>of <i>wcfA</i>-term (BBa_K764010)</p>
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<p><b>**Wednesday 15.8.12**</b></p><br>
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<p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#1d1d1b"><u>Transferred <i>wcfA</i>-term colonies to LB(tet) medium</u></a></p><br>
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<p><b>**Thursday 16.8.12**</b></p><br>
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<p><i>wfcA</i>-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C</p>
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<p>Supernatant discarded</p>
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<p><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57B947" target="_blank"><u>Fermentas protocol</u></a></p>
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<p><b><i>Step 10</i></b>45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation</p>
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<p>Nanodropped for following results: </p><p>
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(A) 329.7 ng/µl</p><p>
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(B) 50.0 ng/µl</p><p>
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(C) 206.6 ng/µl</p><br><p>
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Made streak plates from culture dregs</p>
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Revision as of 17:28, 25 September 2012

ExiGEM2012 Lab Book Gibs wk6

Operon Construction: 13th - 17th August 2012

 **Tuesday 14.8.12**


Transformation of wcfA-term (BBa_K764010)

**Wednesday 15.8.12**


Transferred wcfA-term colonies to LB(tet) medium


**Thursday 16.8.12**


wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C

Supernatant discarded

Fermentas protocol

Step 1045µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation

Nanodropped for following results:

(A) 329.7 ng/µl

(B) 50.0 ng/µl

(C) 206.6 ng/µl


Made streak plates from culture dregs

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