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Team:Nevada/Week 14
From 2012.igem.org
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Leochong718 (Talk | contribs) |
(→August 20) |
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==August 20== | ==August 20== | ||
| + | |||
| + | :Joe: | ||
| + | :::gel 167 - RFP pcr good, gel purify | ||
| + | :::Digestions: SBP(SpeI/PstI), RFP(XbaI/PstI) | ||
| + | :::Ligation of two digestions | ||
| + | |||
| + | :Michelle: | ||
| + | :::Western blot transfer continued from 8/17 for samples of small colony from transformation of M2-BL21 developed :::membrane. | ||
| + | |||
| + | :Justin and Dafne: | ||
| + | :::Large scale expression (200ml) of L-arabinose induced SBP-B12 protein with 5 nM vitamin B-complex | ||
| + | |||
| + | :Jeremiah & Chris: | ||
| + | :::Western blot protocol (BL21 cells with no thiamine added) | ||
| + | :::Run time points on a polyacrylamide gel (BL21 cells with thiamine added) | ||
==August 21== | ==August 21== | ||
Revision as of 03:37, 4 October 2012
Protocols | Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21
Contents |
August 20
- Joe:
- gel 167 - RFP pcr good, gel purify
- Digestions: SBP(SpeI/PstI), RFP(XbaI/PstI)
- Ligation of two digestions
- Michelle:
- Western blot transfer continued from 8/17 for samples of small colony from transformation of M2-BL21 developed :::membrane.
- Justin and Dafne:
- Large scale expression (200ml) of L-arabinose induced SBP-B12 protein with 5 nM vitamin B-complex
- Jeremiah & Chris:
- Western blot protocol (BL21 cells with no thiamine added)
- Run time points on a polyacrylamide gel (BL21 cells with thiamine added)
