Team:NCTU Formosa/Team

From 2012.igem.org

(Difference between revisions)
Line 713: Line 713:
<div class="calcontainer">
<div class="calcontainer">
<div class="calheader">
<div class="calheader">
-
<h2>March 2012</h2>
+
<h2>April 2012</h2>
</div>
</div>
<div class="daysweek">
<div class="daysweek">
Line 728: Line 728:
<div class="week">
<div class="week">
<div class="day">
<div class="day">
-
<div class="daybar"><p>11</p></div>
+
<div class="daybar"><p>1</p></div>
<div class="dots">
<div class="dots">
 +
<li class="caldot yellow"></li>
</div>
</div>
<div class="open">
<div class="open">
 +
<li class="yellow"><p>ligation : insert Zif268 [ES] & AlsS [XP]/Vector pSB1K3 [EP]</p></li>
</div>
</div>
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>12</p></div>
+
<div class="daybar"><p>2</p></div>
<div class="dots">
<div class="dots">
 +
<li class="caldot yellow"></li>
</div>
</div>
<div class="open">
<div class="open">
 +
<li class="yellow"><p>transformation of Zif268+AlsS+pSB1K3 and cultivation on LB-K plate</p></li>
</div>
</div>
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>13</p></div>
+
<div class="daybar"><p>3</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
-
<li class="caldot yellow"></li>
 
</ul>
</ul>
</div>
</div>
Line 755: Line 758:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow"><p>mini-prep of cultivated AlsS, pSB1K3, pSB1A3, and pSB1C3 E.coli  (G2)</p></li>
 
</ul>
</ul>
</div>
</div>
Line 761: Line 763:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>14</p></div>
+
<div class="daybar"><p>4</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 771: Line 773:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>digestion : [Zif268] ES & [AlsS] XP & [pSB1C3] EP (G2)</p></li>
+
<li class="yellow e2"><p>digestion : [pSB1K3] EP</p></li>
-
<li class="yellow e2"><p>ligation : insert[Zif268] ES & [AlsS] XP/Vector[pSB1C3] EP (G2)</p></li>
+
<li class="yellow e2"><p>digestion : [pSB1K3] EP</p></li>
</ul>
</ul>
</div>
</div>
Line 778: Line 780:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>15</p></div>
+
<div class="daybar"><p>5</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 791: Line 793:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>16</p></div>
+
<div class="daybar"><p>6</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
-
<li class="caldot yellow"></li>
 
-
<li class="caldot yellow"></li>
 
</ul>
</ul>
</div>
</div>
Line 801: Line 801:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>Transformation of B0034</p></li>
 
-
<li class="yellow e2"><p>Cultivation of transformed E.coli on LBA plate</p></li>
 
</ul>
</ul>
</div>
</div>
Line 808: Line 806:
</div>
</div>
<div class="day brn">
<div class="day brn">
-
<div class="daybar"><p>17</p></div>
+
<div class="daybar"><p>7</p></div>
<div class="dots">
<div class="dots">
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
</div>
</div>
<div class="open">
<div class="open">
 +
<li class="yellow e4"><p>PCR of insert fragment [Zif268+AlsS] OK</p></li>
 +
<li class="yellow e4"><p>DNA Sequencing OK</p></li>
 +
<li class="yellow e4"><p>ligation : point mutation HivC</p></li>
 +
<li class="yellow e4"><p>TA cloning : point mutation HivC  </p></li>
</div>
</div>
Line 820: Line 826:
<div class="week">
<div class="week">
<div class="day">
<div class="day">
-
<div class="daybar"><p>18</p></div>
+
<div class="daybar"><p>8</p></div>
<div class="dots">
<div class="dots">
</div>
</div>
Line 829: Line 835:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>19</p></div>
+
<div class="daybar"><p>9</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
-
<li class="caldot yellow"></li>
 
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
</ul>
</ul>
Line 839: Line 844:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>Transformation of Zif268+AlsS+pSB1C3 in DH5-alpha  (G2)</p></li>
+
<li class="yellow"><p>Single colony isolation from Zif268+AlsS LB-K plate, and cultivation of in liquid LB-K</p></li>
-
<li class="yellow e2"><p>Point mutation HivC (PCR, DPN1 digest, TA cloning, transform) (G2)</p></li>
+
</ul>
</ul>
</div>
</div>
Line 846: Line 850:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>20</p></div>
+
<div class="daybar"><p>10</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
 +
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
Line 856: Line 861:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>PCR of point mutation HivC (G2)</p></li>
+
<li class="yellow e3"><p>mini-prep of cultivated Zif268+ AlsS E. coli</p></li>
-
<li class="yellow e2"><p>PCR of insert fragment [Zif268+AlsS]  (G2)</p></li>
+
<li class="yellow e3"><p>transformation of point mutation HivC and cultivation on LB-A plate</p></li>
 +
<li class="yellow e3"><p>transformation of B0034 and cultivation on LB-A plate</p></li>
</ul>
</ul>
</div>
</div>
Line 863: Line 869:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>21</p></div>
+
<div class="daybar"><p>11</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
-
<li class="caldot yellow"></li>
 
</ul>
</ul>
</div>
</div>
Line 872: Line 877:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow"><p>electrophoresis of insert fragment [Zif268+AlsS]----NOT OK  (G2)</p></li>
 
</ul>
</ul>
</div>
</div>
Line 878: Line 882:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>22</p></div>
+
<div class="daybar"><p>12</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 888: Line 892:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>Single colony isolation from pLac LBA plate, and cultivation of pLac E.coli in liquid LBA</p></li>
+
<li class="yellow e2"><p>Single colony isolation from HivC LB-A plate, and in liquid LB-A</p></li>
-
<li class="yellow e2"><p>Single colony isolation from pSB1K3 LBK plate, and cultivation of pSB1K3 E.coli in liquid LBK</p></li>
+
<li class="yellow e2"><p>Single colony isolation from B0034 LB-A plate, and in liquid LB-A</p></li>
</ul>
</ul>
</div>
</div>
Line 895: Line 899:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>23</p></div>
+
<div class="daybar"><p>13</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 904: Line 908:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow"><p>mini-prep of cultivated pLac & pSB1K3 E.coli</p></li>
+
<li class="yellow"><p>mini-prep of cultivated point mutation HivC E. coli & B0034 E. coli </p></li>
</ul>
</ul>
</div>
</div>
Line 910: Line 914:
</div>
</div>
<div class="day brn">
<div class="day brn">
-
<div class="daybar"><p>24</p></div>
+
<div class="daybar"><p>14</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 919: Line 923:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow"><p>digestion : [pSB1K3] EP</p></li>
+
<li class="yellow"><p>digestion : Zif268+AlsS [XP]</p></li>
</ul>
</ul>
</div>
</div>
Line 928: Line 932:
<div class="week">
<div class="week">
<div class="day">
<div class="day">
-
<div class="daybar"><p>25</p></div>
+
<div class="daybar"><p>15</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 941: Line 945:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>26</p></div>
+
<div class="daybar"><p>16</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
Line 954: Line 958:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>27</p></div>
+
<div class="daybar"><p>17</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
 +
<li class="caldot yellow"></li>
</ul>
</ul>
</div>
</div>
Line 962: Line 967:
<div class="open">
<div class="open">
<ul>
<ul>
 +
<li class="yellow"><p>Single colony isolation from ilvD LB-A plate, and cultivation in liquid LB-A </p></li>
</ul>
</ul>
</div>
</div>
Line 967: Line 973:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>28</p></div>
+
<div class="daybar"><p>18</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
</ul>
</ul>
</div>
</div>
Line 975: Line 984:
<div class="open">
<div class="open">
<ul>
<ul>
 +
<li class="yellow e3"><p>mini-prep of cultivated ilvD E. coli</p></li>
 +
<li class="yellow e3"><p>digestion : ilvD [EP] & pSB1K3 [EP]</p></li>
 +
<li class="yellow e3"><p>transformation of 37℃ RBS and cultivation on LB-C plate</p></li>
</ul>
</ul>
</div>
</div>
Line 980: Line 992:
</div>
</div>
<div class="day">
<div class="day">
-
<div class="daybar"><p>29</p></div>
+
<div class="daybar"><p>19</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow"><p>Single colony isolation from HivC LB-A plate, and cultivation in liquid LB-A </p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>20</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
Line 990: Line 1,019:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>Single colony isolation from Zif268 LBA plate, and cultivation of Zif268 E.coli in liquid LBA</p></li>
+
<li class="yellow e4"><p>digestion : B0034 [SP]</p></li>
-
<li class="yellow e2"><p>Single colony isolation from AlsS LBA plate, and cultivation of AlsS E.coli in liquid LBA</p></li>
+
<li class="yellow e4"><p>gel extraction</p></li>
 +
<li class="yellow e4"><p>ligation :insert Zif268+AlsS [XP]/Vector B0034 [SP]</p></li>
 +
<li class="yellow e4"><p>mini-prep of cultivated Hivc E. coli</p></li>
 +
</ul>
 +
</div>
 +
 
 +
</div>
 +
<div class="day brn">
 +
<div class="daybar"><p>21</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e2"><p>transformation of B0034+Zif268+AlsS and cultivation on LB-A plate</p></li>
 +
<li class="yellow e2"><p>transformation of HivC and cultivation on LB-A plate</p></li>
</ul>
</ul>
</div>
</div>
</div>
</div>
 +
</div>
 +
<!---------------------------------------- week end ---------------------------------------->
 +
<!---------------------------------------- week start ---------------------------------------->
 +
<div class="week">
<div class="day">
<div class="day">
-
<div class="daybar"><p>30</p></div>
+
<div class="daybar"><p>22</p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e8"><p>PCR of insert fragment [B0034+Zif268+AlsS] OK</p></li>
 +
<li class="yellow e8"><p>DNA Sequencing NOT OK</p></li>
 +
<li class="yellow e8"><p>digestion : B0034 [SP] & Zif268+AlsS [XP]</p></li>
 +
<li class="yellow e8"><p>gel extraction</p></li>
 +
<li class="yellow e8"><p>ligation :insert Zif268+AlsS [XP]/Vector B0034+pSB1A3 [SP]</p></li>
 +
<li class="yellow e8"><p>transformation of B0034+Zif268+AlsS and cultivation on LB-A plate</p></li>
 +
<li class="yellow e8"><p>Single colony isolation from B0034 LB-A plate, and cultivation in liquid LB-A</p></li>
 +
<li class="yellow e8"><p>Single colony isolation from ilvD LB-K plate, and cultivation in liquid LB-K</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>23</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e4"><p>PCR of insert fragment [B0034+Zif268+AlsS] NOT OK</p></li>
 +
<li class="yellow e4"><p>transformation of B0034+Zif268+AlsS and cultivation on LB-A plate</p></li>
 +
<li class="yellow e4"><p>mini-prep of cultivated B0034 and ilvD E. coli</p></li>
 +
<li class="yellow e4"><p>Single colony isolation from HivC LB-A plate, and cultivation in liquid LB-A</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>24</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e2"><p>mini-prep of cultivated HivC E. coli</p></li>
 +
<li class="yellow e2"><p>digestion : B0034 [SP] & ilvD [XP]</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>25</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e3"><p>digestion : pSB1C3 [EP] & HivC [ES] & HivC [SP]</p></li>
 +
<li class="yellow e3"><p>ligation :insert HivC [ES] & ilvD [XP]/Vector pSB1C3 [EP]</p></li>
 +
<li class="yellow e3"><p>ligation :insert ilvD [XP]/Vector HivC+pSB1A3 [SP]</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>26</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow"><p>transformation of HivC+ilvD and cultivation on LB-A & LB-C plate</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>27</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow"><p>PCR of insert fragment [B0034+Zif268+AlsS] NOT OK</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day brn">
 +
<div class="daybar"><p>28</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow e3"><p>transformation of pSB1A3 & pSB1C3 and cultivation on LB-A & LB-C plate</p></li>
 +
<li class="yellow e3"><p>PCR of insert fragment [HivC+ilvD] OK</p></li>
 +
<li class="yellow e3"><p>DNA sequencing NOT OK</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
</div>
 +
<!---------------------------------------- week end ---------------------------------------->
 +
<!---------------------------------------- week start ---------------------------------------->
 +
<div class="week">
 +
<div class="day">
 +
<div class="daybar"><p>29</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow"><p>Single colony isolation from pSB1C3 LB-C plate, and cultivation in liquid LB-C</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p>30</p></div>
 +
<div class="dots">
 +
<ul>
 +
<li class="caldot yellow"></li>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
<li class="yellow"><p>mini-prep of cultivated & pSB1C3 E. coli</p></li>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p></p></div>
 +
<div class="dots">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p></p></div>
 +
<div class="dots">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p></p></div>
 +
<div class="dots">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
<div class="open">
 +
<ul>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
<div class="day">
 +
<div class="daybar"><p></p></div>
 +
<div class="dots">
 +
<ul>
</ul>
</ul>
</div>
</div>
Line 1,007: Line 1,265:
<div class="open">
<div class="open">
<ul>
<ul>
-
<li class="yellow e2"><p>mini-prep of cultivated Zif268 Ecoli & AlsS Ecoli</p></li>
 
-
<li class="yellow e2"><p>digestion :[Zif268] ES/[AlsS] XP</p></li>
 
</ul>
</ul>
</div>
</div>
Line 1,014: Line 1,270:
</div>
</div>
<div class="day brn">
<div class="day brn">
-
<div class="daybar"><p>31</p></div>
+
<div class="daybar"><p></p></div>
<div class="dots">
<div class="dots">
<ul>
<ul>

Revision as of 12:22, 18 September 2012

Team:NCTU Formosa - 2012.igem.org

Back to Top

　 Chang, Ting

Hello everyone! I am Zoe. I love to swim and drawing pictures.I am happy to join in this iGEM group. Many things I have learned from the process. It is truely a good experience to me forever. Zoe is the head of experiment group 1 which are responsible for assembling the first half circuits of our i-butanol-producing enzymes. She is the chief designer of our animation.

　 Hsieh, Hsin-Yun

Hello, She's Lisa Hsieh. She enjoys the process of the experiment with teammates. She likes to draw pictures. With a little perfectism, it took her 2 days to finish team's mascot. She especially likes to drink a cup of hot cocoa after a long day work, and she believes cocoa can cure all diseases! Lisa worked with Zoe and Pearl to construct PSB II + ilvC and Ptet + B0032 + Gli1. She is the designer of our cute robot mascot.

　 Yu, Chun-Wei

Chun-Wei is a 3rd year biotechnology student at NCTU, love nature and playing guitar also love biology. He joined iGEM to get involved in the world-changing field of synthetic biology. Together with Jenny, Chun-Wei assembled the first two part of our enzyme series, and did the tolerance experiment. He is also an important role in our Human practice who trained students to be our human practice staffs.

　 Chen, Wei-Ting

Vicky Chen is a junior in National Chaio Tung University, major in bio-technology. She likes spending time in wet lab, discussing with her teammates. She enjoys eating foods ,shopping and traveling. Vicky assembled zif268 and alsS and optimized the growth condition in which E. coli can produce most i-butanol with Yu-Che.

　 Wu, Cheng-Hsuan

Wu, CHENG-HSUAN (English name: Max) is a third year Biological Science and Technology at NCTU. He enjoys playing basketball and going around the night markets. You can contact CHENG-HSUAN at max810327@hotmail.com.tw Max is the head of experiment group 2 which are responsible for assembling the second half circuits of our i-butanol-producing enzymes. Our instrument is mainly designed by him.

　 Lee, Chia-Jung

As a student majoring in biological science and technology in the third year in NCTU, Chia-Jung always want to know the logic and consequences behind things around him. He likes Chemistry and Biology particularly. In his free time, he likes playing tennis and training to improve his skill. Chia-Jung is the leader of 2012 NCTU-Formosa. He worked with group 2 to assemble biobrick and participated in summer camp as a TA.

　 Yu, Chieh-Cheng

Charlie white yu is a third-year student National Chiao Tung University who is majoring in Biological Science and Technology. He thought iGEM would be a great opportunity to further his knowledge of genetics, gain valuable research experience, and explore possible area of interest. Charlie is in charge of survey jobs and souvenir design. He was a menber of group 2 and also a TA in our summer camp.

　 Lin, I

Hi, my name is Lin I. I am from Taiwan and I major in Biotechnology in National Chiao Tung University. Playing basketball and listening classical music are my hobbies. It is really exciting and pleasant that I can be one of the 2012 iGEM team members. However, this is not all about me. If you have willing to contact me, maybe we will become friend! I is the “pro” of constructing biobricks in group 2. No matter how hard is it, I can built it. He also made our opening clip with Kyle.

　 Peng, Kai-Chun

Hi! I'm Kyle, a third year student contributing in Bio-technology at National Chiao Tung University of Taiwan. I'm interest in planting a variety of carnivorous plants which are so beautiful and enigmatic. I’m looking forward to meeting other school team. I hope it may be a real eye-opener for me. Besides being a member of group 2, Kyle played an important role in our summer camp. He edited handout written by TAs, designed courses, experiments and iGEM lectures. And he is the head of our video team which made our opening clip and animation.

　 Tsai, Lu-An

TSAI, Lu-An is a Qusai-Bachelor of NCTU, the adherent of Adeptus scientia and Preacher of capitalism. He loves the smell of napalm in the morning, the view of chaos in parliament. By using strange logic and weird metaphor, he can confuse the listeners, and Unfortunately himself. Besides being a member of group 2, Lu-An is the head of wiki group which made our beautiful team wiki. He also gave a lecture to students with pearl in summer camp.

　 Chung, Chia-Jung

Hello, I'm Pearl. I like to do experiments, and I go to lab rain or shine even on typhoon. Besides that, I like to chat with friend. I wear a big smile on my face .I like to practice cheerleading, too. Together with Lisa and Zoe, Pearl to construct PSB II + ilvC and Ptet + B0032 + Gli1. And she is the main designer of our T-shirt. She also gave a lecture to students with Lu-An in summer camp.

　 Cheng, Yu-Che

CHENG Yu-Che is a third year biotechnology student at National Chiao-Tung University. He's addicted to synthetic biology and molecular biology. So he may go this way in the future and keep playing table tennis which he is good at. Yu-Che assembled zif268 and alsS and optimized the growth condition in which E. coli can produce most i-butanol with vicky.

　 Soo, Ching-Ren

Soo, Ching-Ren is a Biological Science and Technology at NCTU. He is from Malaysia, hobby is play video games. He enjoys experiment of IGEM, he think it will very usefull in his future. Ching-Ren worked with Lisa Pearl and Zoe to assembled Ptet + B0032 + Gli1 + kivd + B0032

　 Hsu, Tzu-Yun

Jenny Hsu is a third-year student who is majoring in Biotechnology in National Chiao Tung University. She enjoys doing the experiments, cooking, and making handicraft. She thinks that attending 2012 iGEM is a good experience to her, and hopes she can make many friends in iGEM and share knowledge with each other. Jenny assembled the first two part of our enzyme series, and did the tolerance experiment with Chun-Wei. She also played an important role in summer camp. She connected our department for fund and gathered students to make our plan come true.

　 Calvin Hue

I’m a Photonics major at NCTU. I was overwhelmed with fun during our cooperation and learned how practical and systematic Bioscience can be. It’s also my pleasure to get involved in this project and make new friends. At the moment, I'm mainly working on the wiki site. I hope to make it user-friendly and eye-pleasing.

　 Tseng, Ching-Ping

He is a Professor of Biological Science and Technology at National Chiao Tung University, Hsinchu, Taiwan, R.O.C. Highest Education: Ph. D. Rutgers University, USA, 1991.Since 2001, Tseng has been a professor in NCTU, Taiwan. His current research interests include molecular biology, synthetic biology, applied microbiology, and bioengineering.

　 Lee, Hsiao-Ching

Received the B.S. degree and Ph.D. degree from the Department of Life Science at National Tsing Hua University, Hsinchu, Taiwan, R.O.C. in 1999 and 2005 respectively. From 2005 to 2007, she worked as a postdoctoral research position at NTHU. She is now an Assistant Professor with the Department of Biological Science and Technology at National Chiao Tung Universit. Her current research interests include molecular biology, synthetic biology and bioinformatics.

　 Huang, Hsien-Da

Dr. Huang is a Professor and the current Chairman of Department of Biological Science & Technology, Institute of Bioinformatics and Systems Biology of National Chiao Tung University, Hsinchu, Taiwan R.O.C. His current research include microRNA regulation and protein post-translational modification databases building and dry-lab tools development.

　 Yu-Pei Chen

Received the Ph.D. degree from the Department of Biological Science and Technology at National Chiao Tung University, Hsinchu, Taiwan, R.O.C. in 2008. From 2008 to 2012, he worked as a postdoctoral research position in Department of Biological Science and Technology and Mechanical Engineering at NCTU. His current research interests include molecular biology, biochemistry and fungi secondary metabolite.

　 Wu, Shao-I

I am a master student, majoring molecular medicine and bioengineering. Hobby: travel, photography, music, volleyball.

　 Tseng, De-Jung

I’m De-Jung Tseng, a student with plenty of ideas. I believe that our great effort to realize the inspiration would change the world. Be the artist of life. De-Jung design our beautiful souvenir

　 Chang, Shu-Han

As a student majoring in biological science and technology of National Chiao-Tung University, I have the chance to join the competition. Although we have lots of problem to solve, but we never give up and finish the job. Since then, I love my partners so much.

　 Malvin Jefri

An International student in NCTU, majoring in Biological Science and Technology. I'm a badminton freak who loves watching Korean serial movies and Korean boy bands. It's my pleasure to join IGEM competition to taste what's so called teamwork in doing experiment as a team. There's so much sweet n sour moment during these period. Last, I'm just An ordinary Indonesian boy who does ordinary stuffs but always planned to go beyond my limit.

Notes

March 2012

Sunday

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

11

12

13

  • mini-prep of cultivated AlsS, pSB1K3, pSB1A3, and pSB1C3 E.coli (G2)

14

  • digestion : [Zif268] ES & [AlsS] XP & [pSB1C3] EP (G2)

  • ligation : insert[Zif268] ES & [AlsS] XP/Vector[pSB1C3] EP (G2)

15

16

  • Transformation of B0034

  • Cultivation of transformed E.coli on LBA plate

17

18

19

  • Transformation of Zif268+AlsS+pSB1C3 in DH5-alpha (G2)

  • Point mutation HivC (PCR, DPN1 digest, TA cloning, transform) (G2)

20

  • PCR of point mutation HivC (G2)

  • PCR of insert fragment [Zif268+AlsS] (G2)

21

  • electrophoresis of insert fragment [Zif268+AlsS]----NOT OK (G2)

22

  • Single colony isolation from pLac LBA plate, and cultivation of pLac E.coli in liquid LBA

  • Single colony isolation from pSB1K3 LBK plate, and cultivation of pSB1K3 E.coli in liquid LBK

23

  • mini-prep of cultivated pLac & pSB1K3 E.coli

24

  • digestion : [pSB1K3] EP

25

26

27

28

29

  • Single colony isolation from Zif268 LBA plate, and cultivation of Zif268 E.coli in liquid LBA

  • Single colony isolation from AlsS LBA plate, and cultivation of AlsS E.coli in liquid LBA

30

  • mini-prep of cultivated Zif268 Ecoli & AlsS Ecoli

  • digestion :[Zif268] ES/[AlsS] XP

31

April 2012

Sunday

Monday

Tuesday

Wednesday

Thursday

Friday

Saturday

1

  • ligation : insert Zif268 [ES] & AlsS [XP]/Vector pSB1K3 [EP]

    • 2

  • transformation of Zif268+AlsS+pSB1K3 and cultivation on LB-K plate

    • 3

    4

    • digestion : [pSB1K3] EP

    • digestion : [pSB1K3] EP

    5

    6

    7

  • PCR of insert fragment [Zif268+AlsS] OK

  • DNA Sequencing OK

  • ligation : point mutation HivC

  • TA cloning : point mutation HivC

    • 8

    9

    • Single colony isolation from Zif268+AlsS LB-K plate, and cultivation of in liquid LB-K

    10

    • mini-prep of cultivated Zif268+ AlsS E. coli

    • transformation of point mutation HivC and cultivation on LB-A plate

    • transformation of B0034 and cultivation on LB-A plate

    11

    12

    • Single colony isolation from HivC LB-A plate, and in liquid LB-A

    • Single colony isolation from B0034 LB-A plate, and in liquid LB-A

    13

    • mini-prep of cultivated point mutation HivC E. coli & B0034 E. coli

    14

    • digestion : Zif268+AlsS [XP]

    15

    16

    17

    • Single colony isolation from ilvD LB-A plate, and cultivation in liquid LB-A

    18

    • mini-prep of cultivated ilvD E. coli

    • digestion : ilvD [EP] & pSB1K3 [EP]

    • transformation of 37℃ RBS and cultivation on LB-C plate

    19

    • Single colony isolation from HivC LB-A plate, and cultivation in liquid LB-A

    20

    • digestion : B0034 [SP]

    • gel extraction

    • ligation :insert Zif268+AlsS [XP]/Vector B0034 [SP]

    • mini-prep of cultivated Hivc E. coli

    21

    • transformation of B0034+Zif268+AlsS and cultivation on LB-A plate

    • transformation of HivC and cultivation on LB-A plate

    22

    • PCR of insert fragment [B0034+Zif268+AlsS] OK

    • DNA Sequencing NOT OK

    • digestion : B0034 [SP] & Zif268+AlsS [XP]

    • gel extraction

    • ligation :insert Zif268+AlsS [XP]/Vector B0034+pSB1A3 [SP]

    • transformation of B0034+Zif268+AlsS and cultivation on LB-A plate

    • Single colony isolation from B0034 LB-A plate, and cultivation in liquid LB-A

    • Single colony isolation from ilvD LB-K plate, and cultivation in liquid LB-K

    23

    • PCR of insert fragment [B0034+Zif268+AlsS] NOT OK

    • transformation of B0034+Zif268+AlsS and cultivation on LB-A plate

    • mini-prep of cultivated B0034 and ilvD E. coli

    • Single colony isolation from HivC LB-A plate, and cultivation in liquid LB-A

    24

    • mini-prep of cultivated HivC E. coli

    • digestion : B0034 [SP] & ilvD [XP]

    25

    • digestion : pSB1C3 [EP] & HivC [ES] & HivC [SP]

    • ligation :insert HivC [ES] & ilvD [XP]/Vector pSB1C3 [EP]

    • ligation :insert ilvD [XP]/Vector HivC+pSB1A3 [SP]

    26

    • transformation of HivC+ilvD and cultivation on LB-A & LB-C plate

    27

    • PCR of insert fragment [B0034+Zif268+AlsS] NOT OK

    28

    • transformation of pSB1A3 & pSB1C3 and cultivation on LB-A & LB-C plate

    • PCR of insert fragment [HivC+ilvD] OK

    • DNA sequencing NOT OK

    29

    • Single colony isolation from pSB1C3 LB-C plate, and cultivation in liquid LB-C

    30

    • mini-prep of cultivated & pSB1C3 E. coli

    Retrieved from "http://2012.igem.org/Team:NCTU_Formosa/Team"