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Team:Wageningen UR/Journal/week10
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* A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated. | * A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated. | ||
| - | * Because there is iGEM illegal sites in our TuYV readthrough part, we | + | * Because there is iGEM illegal sites in our TuYV readthrough part, we designed a new construct named 4 and 4H, which were coat protein with part of the readthrough without illegal site and coat protein with part of the readthrough without illegal site with his-tag. |
| - | * We tried to isolate and amplify 4 and 4H today, but we did not | + | * We tried to isolate and amplify 4 and 4H today, but we did not get any band on the agrose gel check |
Tuesday: | Tuesday: | ||
| - | * today we successfully PCR amplified the construct 4, which | + | * today we successfully PCR amplified the construct 4, which should be around 800bp. |
[[File:PCR amplification of 4.jpg]] | [[File:PCR amplification of 4.jpg]] | ||
Wednesday: | Wednesday: | ||
| - | * Today we tried another time to PCR amplify the construct 4H and | + | * Today we tried another time to PCR amplify the construct 4H and we got expected band on the gel check . |
[[File:PCR amplification of 4H.jpg]] | [[File:PCR amplification of 4H.jpg]] | ||
Revision as of 08:35, 20 September 2012
week 10: 2 july - 8 july
Office work
Lab work
TuYV
Monday:
- A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated.
- Because there is iGEM illegal sites in our TuYV readthrough part, we designed a new construct named 4 and 4H, which were coat protein with part of the readthrough without illegal site and coat protein with part of the readthrough without illegal site with his-tag.
- We tried to isolate and amplify 4 and 4H today, but we did not get any band on the agrose gel check
Tuesday:
- today we successfully PCR amplified the construct 4, which should be around 800bp.
Wednesday:
- Today we tried another time to PCR amplify the construct 4H and we got expected band on the gel check .
Written by: Han Yue
