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Team:Wageningen UR/Journal/week10
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* A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated. | * A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa ''E. Coli'' was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated. | ||
| + | * Because there is iGEM illegal sites in our TuYV readthrough part, we use a new construct named 4 and 4H, which are coat protein with part of the readthrough without illegal site and coat protein with part of the readthrough without illegal site with his-tag. | ||
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| + | We tried to isolate and amplify 4 and 4H today, but we did not got any bands on the agrose gel check | ||
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| - | Written by: | + | Written by: Han Yue |
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week11 next week]] | [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week11 next week]] | ||
Revision as of 09:24, 18 September 2012
week 10: 2 july - 8 july
Office work
Lab work
TuYV
Monday:
- A new transformation of last weeks ligation mixes into our electrocompetent DH5 Alfa E. Coli was performed. The next day we checked the colony, there were many colonies on the positive control, however there were the same level of colonies on the sample and negative control. The transformation was contaminated.
- Because there is iGEM illegal sites in our TuYV readthrough part, we use a new construct named 4 and 4H, which are coat protein with part of the readthrough without illegal site and coat protein with part of the readthrough without illegal site with his-tag.
We tried to isolate and amplify 4 and 4H today, but we did not got any bands on the agrose gel check
- Two new gene variants were PCR amplified from the TuYV genome, adding iGEM pre- and suffixes and in one case a Histidine tag. Succesful results were acquired after three attempts.
Written by: Han Yue
