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Team:Wageningen UR/Journal/week8
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*With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthroug, later they will be named 1,1H,2,2H,3,3H. | *With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthroug, later they will be named 1,1H,2,2H,3,3H. | ||
| - | + | [[File:P12 PCR amplification of 1,1H, 2, 2H, 3, 3H. .jpg]] | |
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Written by: Han Yue | Written by: Han Yue | ||
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[[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]] | [[https://2012.igem.org/Team:Wageningen_UR/Journal/week7 previous week]] [[https://2012.igem.org/Team:Wageningen_UR/Journal/week9 next week]] | ||
Revision as of 05:06, 18 September 2012
week 8: 18 june - 24 june
Office work
Lab work
TuYV
The DNA of our Polero virus finally arrived! It turns out not to be the Potato Leafroll Virus (PLRV), but instead the Turnip Yellows Virus (TuYV) which is a close relative (also from the Polero genus).
Friday:
- With designed primers, six different constructs were isolated and amplified from TuYV genome, also iGEM prefix and suffix were added to each construct. The six constructs are coat protein of TuYV, coat protein of TuYV with His-tag, Coat protein + 1/2 readthrough, Coat protein + 1/2 readthrough with His-tag, coat protein + readthrough, coat protein + readthroug, later they will be named 1,1H,2,2H,3,3H.
Written by: Han Yue
