"
Team:Evry/Tadpole injection1
From 2012.igem.org
(Difference between revisions)
| Line 9: | Line 9: | ||
We injected 2.3 nl of plasmid at 100ng.µl-1<br/><br/> | We injected 2.3 nl of plasmid at 100ng.µl-1<br/><br/> | ||
<ol> | <ol> | ||
| + | |||
<li> pCS2+ GFP-aid: contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP (Green Fluorescent Protein)(Nishimura et al., 2009).</li>Number of Plasmids injected: ~ 3.78E+7 <br/> <br/> | <li> pCS2+ GFP-aid: contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP (Green Fluorescent Protein)(Nishimura et al., 2009).</li>Number of Plasmids injected: ~ 3.78E+7 <br/> <br/> | ||
| Line 22: | Line 23: | ||
<h2><li>pCS2+ GFP-aid</li> </h2><br/><br/> | <h2><li>pCS2+ GFP-aid</li> </h2><br/><br/> | ||
| + | <h3>24h after injection</h3><br/> | ||
Revision as of 23:48, 17 September 2012
Plasmid injection in fertilized Xenopus laevis eggs
Plasmids injected:
We injected 2.3 nl of plasmid at 100ng.µl-1
- pCS2+ GFP-aid: contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP (Green Fluorescent Protein)(Nishimura et al., 2009). Number of Plasmids injected: ~ 3.78E+7
- pCS2+ citrine: contains the constitutive promoter CMV and the fluorescent protein citrin (yellow). Number of Plasmids injected: ~ 4.38E+7
- pCS2+ mCFP: contains the constitutive promoter CMV and the fluorescent protein mCFP (Cyan Fluorescent Protein). Number of Plasmids injected: ~ 4.37E+7
- pCS2+ GFP-aid co-injected with the plasmid pNHK60 (from Nishimura et al., 2009) : contains the constitutive promoter CMV and the aid sequenced of the aid system fusionned to GFP, pNHK60 contains the CMV promoter followed by the TIR1 protein which in presence of auxin degrade form a complex with aid of GFP-aid and degrade it AID system. Number of Plasmids injected: ~ 1.89E+7 for pCS2+ GFP-aid and ~ 1.3E+7 for pNHK60
- pCS2+ GFP-aid
- pCS2+ citrine
- pCS2+ mCFP
- pCS2+ GFP-aid co-injected with the plasmid pNHK60
24h after injection
References:
Nishimura, K. et al., 2009. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature Methods, 6(12), p.917-922.
