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Team:TU Darmstadt/Materials/LB Bromthymol blue
From 2012.igem.org
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| - | == LB-Agar- | + | == LB-Agar-Bromothymol blue == |
=== About === | === About === | ||
Modification of [https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB plates] for [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue pH screening]. | Modification of [https://2012.igem.org/Team:TU_Darmstadt/Materials/LB LB plates] for [https://2012.igem.org/Team:TU_Darmstadt/Materials/Bromphenol_blue pH screening]. | ||
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* autoclave for 20 min. at 121°C | * autoclave for 20 min. at 121°C | ||
* wait until the temperature decreased under 60°C and add the prefered antibitic | * wait until the temperature decreased under 60°C and add the prefered antibitic | ||
| - | * Add 15 mL of a | + | * Add 15 mL of a bromothymol blue solution |
| - | ** 1 g | + | ** 1 g bromothymol blue |
** 100 mL ethanol | ** 100 mL ethanol | ||
* pour the medium into petri plates (1 L LB-Agar is equal to 40 plates) | * pour the medium into petri plates (1 L LB-Agar is equal to 40 plates) | ||
* store the plates at 4°C | * store the plates at 4°C | ||
Revision as of 20:25, 26 September 2012
LB-Agar-Bromothymol blue
About
Modification of LB plates for pH screening.
Ingredients
- 5 g tryptone
- 10 g yeast extract
- 5 g NaCl
- 15 g agar-agar
- ad. 1 L dist. water
- autoclave for 20 min. at 121°C
- wait until the temperature decreased under 60°C and add the prefered antibitic
- Add 15 mL of a bromothymol blue solution
- 1 g bromothymol blue
- 100 mL ethanol
- pour the medium into petri plates (1 L LB-Agar is equal to 40 plates)
- store the plates at 4°C
