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Team:TU Darmstadt/Materials/Ampicillin
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Ampcillin is a beta-lactam [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria. | Ampcillin is a beta-lactam [https://2012.igem.org/Team:TU_Darmstadt/Materials#Antibiotics antibiotic] that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria. | ||
=== Stock === | === Stock === | ||
| - | # 4 g ampicillin (100 mg/mL) | + | # Mix: |
| - | # ad. 40 mL dist. water | + | #* 4 g ampicillin (100 mg/mL) |
| + | #* ad. 40 mL dist. water | ||
# sterile filtration | # sterile filtration | ||
# aliquot in 1 mL stocks and store at -20°C | # aliquot in 1 mL stocks and store at -20°C | ||
Revision as of 18:13, 16 September 2012
Contents |
Ampicillin
About
Ampcillin is a beta-lactam antibiotic that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria.
Stock
- Mix:
- 4 g ampicillin (100 mg/mL)
- ad. 40 mL dist. water
- sterile filtration
- aliquot in 1 mL stocks and store at -20°C
Stability
- Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
- Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with [http://en.wikipedia.org/wiki/Parafilm Parafilm])
References
- http://en.wikipedia.org/wiki/Ampicillin
