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Team:TU Darmstadt/Materials/Primer Degradation
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| EstA bba without stop codon tag lo || TTTCTTCCTGCAGCGGCCGCTACTAGTATT || Needed to ampflify snythesis product: BsaI-Myctag-EstA-SNP | | EstA bba without stop codon tag lo || TTTCTTCCTGCAGCGGCCGCTACTAGTATT || Needed to ampflify snythesis product: BsaI-Myctag-EstA-SNP | ||
| + | |- | ||
| + | | M13 reverse up || CAGGAAACAGCTATGAC || Needed to get sequnce of the insert of the pEx-6His-tag plasmid | ||
| + | |- | ||
| + | | CalI pIII lo || xxxx || Needed to get sequnce of the insert of pEx-6His-tag plasmid | ||
|} | |} | ||
Revision as of 18:41, 26 September 2012
Primer Degradation
Overview of the primer sequences used for the degradation project.
| Name | Sequnce (5' --> 3') | Information |
|---|---|---|
| BBa EstA down | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAAGTCCAGGCTCAGCGCCA | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa EstA up | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGGCTCCTTCGCCCTATTCGAC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa FsC down | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAGCAGAACCACGGACAGCCC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa FsC up | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCTGCCTACTTCTAACCCTGCC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa PhoA down | GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTAGGCCGGCTGGGCCGCTTTTG | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa PhoA up | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAAACAAAGCACTATTGCACTGGCAC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa pNB-Est13 down | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAATCTGCGGGTTCGGGGCTGTC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| BBa pNB-Est13 up | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTATGATACAACTGTCGAAACACGCTTC | Adds BioBrick compatible sites (up: EcoRI, NotI, XbaI / down: SpeI, NotI, PstI |
| SKV a1 lo Ndel | CGCGCGCATATGGGCCGGCTGGGCCGCTTTTG | Adds restriction site to PhoA |
| SKV a1 up Xbal | GCGCGCTCTAGAATGTTTACACTTTATGCTTCCG | Adds restriction site to PhoA |
| SKV b1 lo Ncol | CGCGCGCCATGGAATCTGCGGGTTCGGGGCTG | Adds restriction site to Est13 |
| SKV b1 up Ndel | GCGCGCCATATGCATCACCATCACCACATGTATGATACAACTGTCGAAACACG | Adds restriction site to Est13 |
| SKV b2 lo Ncol | CGCGCGCCATGGAGCAGAACCACGGACAGCCC | Adds restriction site to FsC |
| SKV b2 up Ndel | GCGCGCCATATGCATCACCATCACCACATGCTGCCTACTTCTAACCCTGC | Adds restriction site to FsC |
| SKV c1 lo EcoRl | CGCGCGGAATTCCTATCAGAAGTCCAGGCTCAGCGCCAGG | Adds restriction site to EstA |
| SKV c1 up Ncol | GCGCGCCCATGGGCTCCTTCGCCCTATTCGACGC | Adds restriction site to EstA |
| SOE A up | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTTACACTTTATGCTTCCGGCTCGTATG | Adds BioBrick prefix to PhoA |
| SOE a1 lo | TGTATCATACATGTGATGATGGTGGTGATGGGCCGGCTGGGCCGCTTTTG | 20 bp overlapp PhoA His6 10 bp overlapp Est13 |
| SOE a2 lo | AGTAGGCAGCATGTGATGATGGTGGTGATGGGCCGGCTGGGCCGCTTTTG | 20 bp overlapp PhoA His6 and 10 bp overlapp FsC |
| SOE b1 lo | GTCGAATAGGGCGAAGGAGCGCCAATCTGCGGGTTCGGGG | 20 bp overlapp EstA and Est13 |
| SOE b1 up | GCCCAGCCGGCCCATCACCACCATCATCACATGTATGATACAACTGTCGA | 12 bp overlapp PhoA His6 und 20 bp overlapp Est13 |
| SOE b2 lo | GTCGAATAGGGCGAAGGAGCAGCAGAACCACGGACAGCCC | 20 bp overlapp EstA and FsC |
| SOE b2 up | GCCCAGCCGGCCCATCACCACCATCATCACATGCTGCCTACTTCTAACCC | 12 bp overlapp PhoA His6 and 20 bp overlapp FsC |
| SOE c1 up | CCCCGAACCCGCAGATTGGCGCTCCTTCGCCCTATTCGAC | 20 bp overlapp Est13 and EstA |
| SOE c2 up | GGGCTGTCCGTGGTTCTGCTGCTCCTTCGCCCTATTCGAC | 20 bp overlapp FsC und EstA |
| SOE D lo | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGAAGTCCAGGCTCAGCGCC | 20 overlapp EstA and adds BioBrick suffix |
| SOE Est13 mut lo | CAATATCGAGAATCCTTAAGAA | Mutates PstI site in Est13 |
| SOE Est13 mut up | CGATAAGCTTGCGAAAGCTGCACCAGAAAGATTCTTAAGGATTCTCGATA | Mutates PstI site in Est13 |
| SOE EstA PstI out up | CGTGGTCTCCCTACAGCAGGCCGGCGCGCGCTAC | Mutates PstI site in EstA |
| SOE EstA PstI out lo | CCACGATGTAGCGCGCGCCGGCC | Mutates PstI site in EstA |
| pEX FsC His SfiI up | GCGCGCGGCCCAGCCGGCCCATCACCACCATCATCACATGCTGCCTACTTCTAACCC | Adds SfiI site to ligate into a pEx expression system |
| pEX FsC Stop SfiI lo | CGCGCGGGCCCCCGAGGCCGTTATCAAGCAGAACCACGGACAGCCCG | Adds SfiI site to ligate into a pEx expression system |
| XbaI Rbs Phoa up | GCGCGCTCTAGAGTTCACACAGGAAACCTACTAGATGAAACAAAGCACTATTGC | Adds RBS to synthesis product: ENX-PhoA-His6tag-pNBEst13-Myctag-EstA-SNP |
| Est13 Bsa1 lo | GCGCGCGGTCTCCAATCTGCGGGTTCGGGGCT | Needed to amplify synthesis product ENX-PhoA-histag-pNBEst13-BsaI |
| EstA Bsa1 up | GCGCGCGGTCTCAGATTGAACAAAAACTAATA | Needed to ampflify snythesis product: BsaI-Myctag-EstA-SNP |
| EstA bba without stop codon tag lo | TTTCTTCCTGCAGCGGCCGCTACTAGTATT | Needed to ampflify snythesis product: BsaI-Myctag-EstA-SNP |
| M13 reverse up | CAGGAAACAGCTATGAC | Needed to get sequnce of the insert of the pEx-6His-tag plasmid |
| CalI pIII lo | xxxx | Needed to get sequnce of the insert of pEx-6His-tag plasmid |
