Team:TU Darmstadt/Protocols/Ammonium sulfate precipitation
From 2012.igem.org
(Difference between revisions)
(→Ammonium sulfate precipitation) |
|||
Line 39: | Line 39: | ||
<!-- end #menu --> | <!-- end #menu --> | ||
</html> | </html> | ||
- | == Ammonium | + | == Ammonium acetate / Ethanol precipitation == |
=== About === | === About === | ||
- | Ammonium | + | Ammonium acetate precipitation is a method used to purify [https://2012.igem.org/Team:TU_Darmstadt/DNA#DNA DNA] by altering their solubility. It is a specific case of a gerneral technique known as [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out salting out]. |
=== Materials === | === Materials === | ||
==== Equipment ==== | ==== Equipment ==== |
Revision as of 14:57, 26 September 2012
Contents |
Ammonium acetate / Ethanol precipitation
About
Ammonium acetate precipitation is a method used to purify DNA by altering their solubility. It is a specific case of a gerneral technique known as salting out.
Materials
Equipment
- -40°C freezer
- Centrifuge (cooling cababilities required!)
- Heat block
Chemicals & consumables
- Ammonium acetate 7M
- 97% EtOH
- Eppis
- ddH2O
Procedure
- add 1/10 volume of 7M ammonium acetate to DNA solution
- add 3 volumes 97% EtOH to DNA solution
- precipitate for 2 hours at -20°C
- centrifuge for 30 min at 13000 rpm and 4°C
- discard supernatant
- dry pellet for 30 min at 50°C
- solve pellet in 30 µL water
Notes
- Important: Cooling must be maintained at all times.
References
- Praktikumsskript Biotechnologie 2012 TU Darmstadt