Team:SDU-Denmark/labwork/Protocols/3A

From 2012.igem.org

(Difference between revisions)
Line 281: Line 281:
<li>PCR step; see PCR protocol</li>
<li>PCR step; see PCR protocol</li>
<li>Digestion</li>
<li>Digestion</li>
-
<blockquote><i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i></blockquote>
+
<i>BSA is only needed for conventional restriction enzymes, NOT fast digest</i>
<ol>
<ol>
  <li>Digest step for Plasmid Backbone</li>
  <li>Digest step for Plasmid Backbone</li>

Revision as of 20:49, 15 September 2012

iGEM TEAM ::: SDU-DENMARK courtesy of NIAID


mRNA Isolation PCR Miniprep Check Digest
3A-Assembly Content Content Content
Content Content Content Content

3A-Assembly -Ligation of 2 parts into a vector

  1. PCR step; see PCR protocol
  2. Digestion
    3. BSA is only needed for conventional restriction enzymes, NOT fast digest
    1. Digest step for Plasmid Backbone

  • Retrieved from "http://2012.igem.org/Team:SDU-Denmark/labwork/Protocols/3A"