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Team:TU Darmstadt/Materials/tctB162
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Latest revision as of 20:50, 26 September 2012
Contents |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]
Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.
tctB162 BioBrick
5' BioBrick Annealing 3' fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc
tctB162 Pst1
Primers for the elimination of a Pst1 site inside the tctB162 BioBrick.
5' 3' fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
tctB162 RBS
Primers for the insertion of a RBS based on the sequence of [http://partsregistry.org/wiki/index.php/Part:BBa_J61100 BBa_J61100] and [http://partsregistry.org/wiki/index.php/Part:BBa_J61101 BBa_J61101].
5' 3' fwd: GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGAAAGAGGGGACAAtactag atgaaaatcaagagccaaaaagactttttctc rev: gtttcttcctgcagcggccgctactagtaGGGTCCTGTCTTTCttagccagcgatgaagctaggcc
