Team:Osaka/week7

From 2012.igem.org

(Difference between revisions)
Line 7: Line 7:
# Electrophoresis
# Electrophoresis
# Tolerance assay of 144,147(MMC)
# Tolerance assay of 144,147(MMC)
-
# To liquid culture(144,145,160)
+
# Transfer to liquid culture(144,145,160)
Line 18: Line 18:
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
#* 160(K):157(upstream)+158(downstream)+1-5A(vector)
# Transformation of ligation products
# Transformation of ligation products
-
# To liquid culture(146,148)
+
# Transfer to liquid culture(146,148)
# Tolerance assay of 144,145(MMC)
# Tolerance assay of 144,145(MMC)
Line 46: Line 46:
# Tolerance assay of 146,148
# Tolerance assay of 146,148
# Transformation of ligation products:161
# Transformation of ligation products:161
-
# To liquid culture(144~148,162)
+
# Transfer to liquid culture(144~148,162)
Line 54: Line 54:
# Tolerance assay (Preliminary test of hydrogen peroxide)
# Tolerance assay (Preliminary test of hydrogen peroxide)
# Gel electrophoresis of digests
# Gel electrophoresis of digests
-
# To liquid culture(161)
+
# Transfer to liquid culture:149,152,161
==September 15 (Sat)==
==September 15 (Sat)==
Line 66: Line 66:
#* 165(C):162(upstream)+151(downstream)+1-3A(vector)
#* 165(C):162(upstream)+151(downstream)+1-3A(vector)
#* 166(C):162(upstream)+152(downstream)+1-3A(vector)
#* 166(C):162(upstream)+152(downstream)+1-3A(vector)
-
# Transformation of ligation products
+
# Transformation of ligation products into DH5α <i>E.coli</i>
# Tolerance assay (Hydrogen peroxide)
# Tolerance assay (Hydrogen peroxide)
-
 
+
# Transfer to liquid culture:
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Revision as of 04:47, 15 September 2012


September 9 (Sun)

  1. Miniprep of yesterday's culture(150,151,152,159,160)
  2. Restriction digests of minipreppped parts
  3. Electrophoresis
  4. Tolerance assay of 144,147(MMC)
  5. Transfer to liquid culture(144,145,160)


September 10 (Mon)

  1. Miniprep of yesterday's culture(160,1-9N)
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Preparation of LB agar plates(Amp)
  5. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  6. Transformation of ligation products
  7. Transfer to liquid culture(146,148)
  8. Tolerance assay of 144,145(MMC)


September 11 (Tue)

  1. Restriction digests(157,158,159,160,1-9N)
  2. Gel electrophoresis of digests
  3. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  4. Transformation of ligation products
  5. Tolerance assay of 146,148(MMC)


September 12 (Wed)

  1. Miniprep of yesterday's culture:160
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Tolerance assay
  5. Ligation
    • 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
    • 162(C):159(upstream)+160(downstream)+1-3A(vector)

September 13 (Thu)

  1. Colony check
    • 161:Transformed cells didnot grow
  2. Sequencing(157,158,159,160)
  3. Tolerance assay of 146,148
  4. Transformation of ligation products:161
  5. Transfer to liquid culture(144~148,162)


September 14 (Fri)

  1. Miniprep of yesterday's culture:162
  2. Restriction digests of minipreppped parts
  3. Tolerance assay (Preliminary test of hydrogen peroxide)
  4. Gel electrophoresis of digests
  5. Transfer to liquid culture:149,152,161

September 15 (Sat)

  1. Miniprep of yesterday's culture:161
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Gel extraction :161,162
  5. Ligation
    • 163(C):161(upstream)+151(downstream)+1-3A(vector)
    • 164(C):161(upstream)+152(downstream)+1-3A(vector)
    • 165(C):162(upstream)+151(downstream)+1-3A(vector)
    • 166(C):162(upstream)+152(downstream)+1-3A(vector)
  6. Transformation of ligation products into DH5α E.coli
  7. Tolerance assay (Hydrogen peroxide)
  8. Transfer to liquid culture:


Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week7"